Bergmann M, Nashan D, Nieschlag E
Institute of Anatomy, University of Münster, Federal Republic of Germany.
Cell Tissue Res. 1989 Apr;256(1):183-90. doi: 10.1007/BF00224733.
The pattern of compartmentation of the seminiferous epithelium was investigated, using a lanthanum tracer technique, in human testicular biopsies of adult infertile men (age 27 to 44 years), where dislocation of spermatogonia from the basal lamina occurred. Spermatogonia type A and B were found in a two- or three-layered arrangement, in aberrant locations throughout the seminiferous epithelium, and in intratubular positions associated with fragments of Sertoli cell cytoplasm. Tracer impregnation was found around spermatogonia in a multilayered arrangement, indicating the extension of the basal compartment in a luminal direction. Single spermatogonia within the second or third layer of the seminiferous epithelium were regularly found to be surrounded by tracer. The junctional complex between the lateral membranes of adjacent Sertoli cells was devoid of tight junctions. Tracer penetration around spermatogonia in a more luminal position was prevented by intact Sertoli cell junctional complexes; tracer was also absent from intraluminal located spermatogonia associated with cytoplasmic fragments of Sertoli cells. The luminal extension of the basal compartment associated with the dislocation of spermatogonia clearly differs from the pattern of compartmentation during the movement of primary spermatocytes within undisturbed epithelium. There is a strong incidence of elevated serum levels of follicle-stimulating hormone (greater than 7 U/l), indicating a suppression of Sertoli cell function; this may be the cause for the dislocation of spermatogonia and the changes of compartmentation.
利用镧示踪技术，对成年不育男性（年龄27至44岁）的睾丸活检组织进行研究，观察生精上皮的分隔模式，这些患者存在精原细胞从基膜脱位的情况。发现A型和B型精原细胞呈两层或三层排列，位于生精上皮的异常位置以及与支持细胞胞质碎片相关的管内位置。在多层排列的精原细胞周围发现示踪剂浸渍，表明基底小室向管腔方向延伸。在生精上皮第二层或第三层内的单个精原细胞经常被示踪剂包围。相邻支持细胞侧膜之间的连接复合体缺乏紧密连接。完整的支持细胞连接复合体阻止示踪剂渗透到管腔位置更靠内的精原细胞周围；与支持细胞胞质碎片相关的管腔内精原细胞周围也没有示踪剂。与精原细胞脱位相关的基底小室向管腔的延伸明显不同于初级精母细胞在未受干扰的上皮内移动时的分隔模式。促卵泡激素血清水平升高（大于7 U/l）的发生率很高，表明支持细胞功能受到抑制；这可能是精原细胞脱位和分隔变化的原因。
