Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas, N0. 46, Hermosillo, Sonora 83304, Mexico.
Departamento de Investigación en Física, Universidad de Sonora, J, Calle Av. Rosales, Centro, 83000 Hermosillo, Sonora, Mexico.
J Immunol Methods. 2021 Feb;489:112911. doi: 10.1016/j.jim.2020.112911. Epub 2020 Nov 10.
Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is considered a promising vaccination target. The aim of this study was to produce a fully chimeric mouse x pig anti-porcine DEC205 recombinant antibody (rAb). Based on a mouse anti-porcine DEC205 monoclonal antibody (mAb), we designed and expressed a chimeric mouse x pig rAb using the Expi293f system. The resulting rAb maintained the recognition capacity of the native mouse mAb toward the porcine DEC205 receptor, as evidenced by western blot analysis. By using flow cytometry, we evaluated the ability of the rAb to recognize DEC205 dendritic cells. In conclusion, the chimeric mouse x pig anti-DEC205 rAb can be used in antigen-targeting assays as a vaccination strategy in pigs.
重组杂交抗体常用于抗原靶向测定中，以降低在其他物种中使用经典鼠抗体相关的免疫原性。DEC205 受体因其在激活免疫反应方面的有效性而成为一个有吸引力的靶标，被认为是一种很有前途的疫苗接种靶标。本研究旨在制备完全嵌合的鼠 x 猪抗猪 DEC205 重组抗体（rAb）。基于一种鼠抗猪 DEC205 单克隆抗体（mAb），我们使用 Expi293f 系统设计并表达了一种嵌合鼠 x 猪 rAb。Western blot 分析表明，所产生的 rAb 保持了对猪 DEC205 受体的天然鼠 mAb 的识别能力。通过流式细胞术，我们评估了 rAb 识别 DEC205 树突状细胞的能力。总之，嵌合鼠 x 猪抗 DEC205 rAb 可用作抗原靶向测定中的试剂，作为猪的疫苗接种策略。
