J Biomed Nanotechnol. 2020 Jun 1;16(6):954-964. doi: 10.1166/jbn.2020.2933.
infection (CDI) has become the main cause of diarrhea-related diseases in domestic (China) inpatients. High-sensitivity and high-specificity detection methods for CDI must be applied clinically for CDI supervisory control. In this paper, we introduce a detection method for and Norovirus based on real-time PCR. We developed and optimized a primer-probe for Norovirus targets tcdA and tcdB with remarkably increased detection sensitivity. We then used this method in an integrated cassette, and found increased detection efficiency for Norovirus standards in the cassette compared to samples. These results provide a basis for further exploration of automatic testing system design.
艰难梭菌感染（CDI）已成为国内（中国）住院患者腹泻相关疾病的主要原因。必须在临床上应用高灵敏度和高特异性的 CDI 检测方法对 CDI 进行监管控制。在本文中，我们介绍了一种基于实时 PCR 的检测 和诺如病毒的方法。我们针对诺如病毒的靶标 tcdA 和 tcdB 设计并优化了一个引物探针，显著提高了检测的灵敏度。然后，我们在一个集成盒中使用这种方法，发现与 样本相比，该盒中诺如病毒标准品的检测效率有所提高。这些结果为进一步探索自动测试系统设计提供了依据。
