一种用于检测单纯疱疹病毒的高特异性分型方法的开发。
Development of a high specificity typing method for the detection of herpes simplex virus.
作者信息
Chen Zhu, Zhao Kaixuan, Tan Boyu, Tong Zengrui, He Ziyu, Luo Xiaofang, Cai Lei, Wang Hanming, Leung Polly H M, Chow Franklin Wang-Ngai, Chen Hui, Deng Yan
机构信息
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, China.
Department of Scientific Research, Zhuzhou Hospital Affiliated to Xiangya School of Medical, Central South University, Zhuzhou, China.
出版信息
Front Bioeng Biotechnol. 2022 Aug 17;10:955713. doi: 10.3389/fbioe.2022.955713. eCollection 2022.
Herpes disease is caused by Herpes simplex virus (HSV). It has become one of the global health problems. This paper reports a method for HSV type testing. First specific primers sequence for HSV-1 and HSV-2 were selected, designed, and synthesized. Then, these amplification products were proved by sequencing and analysis. Lastly, we optimized the reaction system and PCR reaction program by orthogonal design and sensitivity testing. Results showed that the lowest concentration in HSV-type testing is about 6.67 × 10 copies/ml. Moreover, the specificity of detection was very high. So, this method has very great potentials for HSV type testing in clinical practice.
疱疹疾病由单纯疱疹病毒（HSV）引起。它已成为全球健康问题之一。本文报道了一种HSV分型检测方法。首先，选择、设计并合成了HSV-1和HSV-2的特异性引物序列。然后，通过测序和分析对这些扩增产物进行验证。最后，通过正交设计和灵敏度测试优化了反应体系和PCR反应程序。结果表明，HSV分型检测的最低浓度约为6.67×10拷贝/毫升。此外，检测的特异性非常高。因此，该方法在临床实践中进行HSV分型检测具有很大的潜力。
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