蛋白激酶C在葡萄糖刺激的胰岛素分泌中作用的重新评估。
A re-assessment of the role of protein kinase C in glucose-stimulated insulin secretion.
作者信息
Hii C S, Jones P M, Persaud S J, Howell S L
机构信息
Department of Physiology, Kings College London, University of London, U.K.
出版信息
Biochem J. 1987 Sep 1;246(2):489-93. doi: 10.1042/bj2460489.
Abstract
Isolated rat islets of Langerhans which had been pretreated with 200 nM-phorbol 12-myristate 13-acetate (PMA) for 20-24 h, a treatment reported in other cell types to deplete cells of protein kinase C activity, were found not to contain detectable Ca2+/phospholipid-dependent protein kinase activity. These islets did not secrete insulin in response to a subsequent exposure to PMA (0.1 or 1 microM) during a 30 min incubation, although insulin secretion could be stimulated by 20 mM-glucose, a response which was enhanced by 20 microM-forskolin. PMA-pretreated islets that had been permeabilized by high-voltage discharge showed unimpaired secretory responses to an increase in Ca2+ concentration, cyclic AMP and forskolin. These results suggest that (i) pretreatment of islets with tumour-promoting phorbol esters may be a useful means of investigating the role of protein kinase C in stimulus-secretion coupling in the pancreatic beta-cell and (ii) protein kinase C may not play an essential role in glucose-induced insulin secretion.
摘要
用200 nM佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA）预处理20 - 24小时的分离大鼠胰岛，在其他细胞类型中这种处理会耗尽细胞的蛋白激酶C活性，结果发现这些胰岛不含可检测到的Ca2+/磷脂依赖性蛋白激酶活性。在30分钟的孵育期间，这些胰岛在随后暴露于PMA（0.1或1 microM）时不分泌胰岛素，尽管20 mM葡萄糖可刺激胰岛素分泌，20 microM佛司可林可增强这种反应。经高压放电透化处理的PMA预处理胰岛对Ca2+浓度增加、环磷酸腺苷和佛司可林表现出未受损的分泌反应。这些结果表明：（i）用促肿瘤佛波醇酯预处理胰岛可能是研究蛋白激酶C在胰腺β细胞刺激 - 分泌偶联中作用的有用方法；（ii）蛋白激酶C可能在葡萄糖诱导的胰岛素分泌中不发挥重要作用。
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本文引用的文献
1
Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).使用固相氧化剂1,3,4,6-四氯-3α,6α-二苯基甘脲（碘苷）对蛋白质、糖蛋白和肽进行碘化。
Anal Biochem. 1981 Oct;117(1):136-46. doi: 10.1016/0003-2697(81)90703-x.
2
Phospholipid-sensitive Ca2+-dependent protein kinase from heart. II. Substrate specificity and inhibition by various agents.心脏中的磷脂敏感钙依赖性蛋白激酶。II. 底物特异性及多种试剂的抑制作用
J Biol Chem. 1982 Jul 25;257(14):8489-95.
3
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.肿瘤促进剂佛波酯对钙激活的、磷脂依赖性蛋白激酶的直接激活作用。
J Biol Chem. 1982 Jul 10;257(13):7847-51.
4
Polyamines inhibit phospholipid-sensitive and calmodulin-sensitive Ca2+-dependent protein kinases.多胺抑制磷脂敏感性和钙调蛋白敏感性钙依赖性蛋白激酶。
Biochem J. 1983 Aug 1;213(2):281-8. doi: 10.1042/bj2130281.
5
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Biochem Biophys Res Commun. 1983 Oct 31;116(2):555-62. doi: 10.1016/0006-291x(83)90559-4.
6
Effects of nutrient secretagogues upon phospholipid metabolism in rat pancreatic islets.营养促分泌素对大鼠胰岛磷脂代谢的影响。
Mol Cell Endocrinol. 1983 Oct;32(2-3):205-14. doi: 10.1016/0303-7207(83)90083-7.
7
Activation of protein kinase C by a tumor-promoting phorbol ester in pancreatic islets.肿瘤促进性佛波酯在胰岛中激活蛋白激酶C。
FEBS Lett. 1984 May 21;170(2):247-53. doi: 10.1016/0014-5793(84)81322-8.
8
Polymyxin B is a more selective inhibitor for phospholipid-sensitive Ca2+-dependent protein kinase than for calmodulin-sensitive Ca2+-dependent protein kinase.多粘菌素B对磷脂敏感的钙依赖性蛋白激酶的抑制作用比对钙调蛋白敏感的钙依赖性蛋白激酶更具选择性。
Biochem Biophys Res Commun. 1982 Dec 31;109(4):1129-33. doi: 10.1016/0006-291x(82)91894-0.
9
Insulinotropic effect of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate in rat pancreatic islets.肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯对大鼠胰岛的促胰岛素分泌作用。
Cancer Res. 1980 Oct;40(10):3827-31.
10
Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells.大鼠胰岛和仓鼠β细胞中钙磷脂依赖性蛋白激酶的鉴定与特性分析
Biochem J. 1984 Apr 15;219(2):547-51. doi: 10.1042/bj2190547.
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