大鼠胰岛和仓鼠β细胞中钙磷脂依赖性蛋白激酶的鉴定与特性分析
Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells.
作者信息
Lord J M, Ashcroft S J
出版信息
Biochem J. 1984 Apr 15;219(2):547-51. doi: 10.1042/bj2190547.
Abstract
We show that extracts of rat islets of Langerhans and of cloned hamster beta-cells (HIT-T15 cells) contain Ca2+-phospholipid-dependent protein kinase and endogenous protein substrates for the kinase. We purified Ca2+-phospholipid-dependent protein kinase from HIT-T15 beta-cells and report here its physical and kinetic properties.
摘要
我们发现，大鼠胰岛提取物以及克隆的仓鼠β细胞（HIT-T15细胞）提取物中含有Ca2+ - 磷脂依赖性蛋白激酶及其内源性蛋白底物。我们从HIT-T15β细胞中纯化了Ca2+ - 磷脂依赖性蛋白激酶，并在此报告其物理和动力学特性。
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