Bergère J L, Hayes H, Commissaire J
Laboratoire de Microbiologie Laitière, INRA, Jouy en Josas, France.
Ann Inst Pasteur Microbiol. 1986 Nov-Dec;137B(3):271-82. doi: 10.1016/s0769-2609(86)80117-x.
The overall composition of the Clostridium tyrobutyricum cell envelope did not vary significantly during cell growth and was characterized by a high protein content (about 40% dry weight). Teichoic and teichuronic acids were absent and the neutral sugar content low. Insoluble peptidoglycan represented only 10-12% of the cell envelope (dry weight basis); it contained glucosamine, muramic acid, alanine, diaminopimelic acid and glutamic acid (molecular ratio 1/1/2/1/1). SDS-PAGE revealed the presence of about 50 proteins in this cell envelope; however, one high molecular weight protein was largely predominant. They were not covalently bound to the peptidoglycan and their relative amounts were practically constant through cell growth and with various extraction treatments. A brief heat treatment of whole cells in PBS caused selective release of the major cell envelope proteins together with flagellin; this method was used to characterize these proteins in 37 strains of C. tyrobutyricum and some other clostridia. The major envelope proteins had molecular weights ranging from 96 to 145 Kd and the flagellins from 32 to 72 Kd.
酪丁酸梭菌细胞壁的总体组成在细胞生长过程中变化不显著，其特点是蛋白质含量高（约占干重的40%）。壁磷壁酸和磷壁醛酸不存在，中性糖含量低。不溶性肽聚糖仅占细胞壁的10 - 12%（以干重计）；它含有葡萄糖胺、胞壁酸、丙氨酸、二氨基庚二酸和谷氨酸（分子比为1/1/2/1/1）。SDS - PAGE显示该细胞壁中存在约50种蛋白质；然而，一种高分子量蛋白质占主导地位。它们不与肽聚糖共价结合，其相对含量在细胞生长过程中以及经过各种提取处理后基本保持恒定。在PBS中对全细胞进行短暂热处理会导致主要细胞壁蛋白与鞭毛蛋白一起选择性释放；该方法用于对37株酪丁酸梭菌和其他一些梭菌中的这些蛋白质进行表征。主要的包膜蛋白分子量在96至145 Kd之间，鞭毛蛋白分子量在32至72 Kd之间。
