Hebeler B H, Wong W, Morse S A, Young F E
Infect Immun. 1979 Feb;23(2):353-9. doi: 10.1128/iai.23.2.353-359.1979.
Treatment of cells grown to exponential phase with 4% sodium dodecyl sulfate for 3 h at 100 degrees C resulted in solubilization of all cellular components except for peptidoglycan. In most strains, cells cultured in liquid gonococcal broth at pH 7.2 yielded a peptidoglycan composed primarily of N-acetylmuramic acid N-acetylglucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1:1:2:1:1. The peptidoglycan in these cells accounted for 1 to 2% (dry weight) of the cells. However, in cells cultured at pH 6.0, the dry weight of peptidoglycan increased to 4 to 13%. Preliminary investigations indicated that the apparent increase in weight is strain dependent and is due in part to associated protein(s). Neisseria gonorrhoeae strain CS7 had elevated amounts of protein associated with the peptidoglycan regardless of growth pH. The peptidoglycan-protein complex could not be dissociated by additional extraction with sodium dodecyl sulfate, 10 M LiCl2, or ethylenediaminetetraacetate or by 7.5% polyacrylamide gel electrophoresis. The complex could be degraded by lysozyme, trypsin, chymotrypsin, Pronase B, and Chalaropsis sp. muramidase.
将处于指数生长期的细胞在100℃下用4%的十二烷基硫酸钠处理3小时，结果除肽聚糖外所有细胞成分均被溶解。在大多数菌株中，在pH 7.2的液体淋球菌肉汤中培养的细胞产生的肽聚糖主要由N - 乙酰胞壁酸、N - 乙酰葡糖胺、丙氨酸、谷氨酸和二氨基庚二酸组成，摩尔比为1:1:2:1:1。这些细胞中的肽聚糖占细胞干重的1%至2%。然而，在pH 6.0条件下培养的细胞中，肽聚糖的干重增加到4%至13%。初步研究表明，重量的明显增加取决于菌株，部分原因是相关蛋白质。淋病奈瑟菌CS7菌株无论生长pH如何，与肽聚糖相关的蛋白质含量都升高。肽聚糖 - 蛋白质复合物不能通过用十二烷基硫酸钠、10 M LiCl₂或乙二胺四乙酸进一步提取或通过7.5%聚丙烯酰胺凝胶电泳解离。该复合物可被溶菌酶、胰蛋白酶、糜蛋白酶、链霉蛋白酶B和Chalaropsis sp. 溶菌酶降解。
