基于自组装非结构蛋白 1 的纳米疫苗增强了对寨卡病毒的抗体反应。

Nanovaccine based on self-assembling nonstructural protein 1 boosts antibody responses to Zika virus.

机构信息

Vaccine Development Laboratory, Microbiology Department, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

出版信息

Nanomedicine. 2021 Feb;32:102334. doi: 10.1016/j.nano.2020.102334. Epub 2020 Nov 11.

DOI:10.1016/j.nano.2020.102334

PMID:33188909

Abstract

Self-assembling proteins may be generated after the addition of short specific amino acid sequences at both the N- and C-terminal ends. To date, this approach has not been evaluated regarding the impact of self-assembled proteins on the induction of immune responses. In the present study, we report the application of this experimental approach to the immunogenicity of protein antigens by measuring the antibody responses in mice immunized with nanoparticles made with a recombinant form of Zika virus nonstructural protein 1 (∆NS1). The results clearly indicated that ∆NS1-derived nanoparticles (NP-∆NS1) are assembled into a 3-dimensional structure with a high degree of multimerization. While ∆NS1 proved to be a weak immunogen, immunization with NP-∆NS1 enhanced subunit vaccines' immunogenicity with improved longevity in vaccinated mice. Thus, immunization with self-assembled antigens (nanovaccines) represents a new and promising strategy to enhance NS1-specific antibodies' induction based on purified recombinant proteins.

摘要

自组装蛋白可以在 N 端和 C 端添加短的特定氨基酸序列后产生。迄今为止，尚未评估这种方法对自组装蛋白诱导免疫反应的影响。在本研究中，我们通过测量用重组 Zika 病毒非结构蛋白 1（∆NS1）制成的纳米颗粒免疫的小鼠的抗体反应，报告了该实验方法在蛋白抗原免疫原性中的应用。结果清楚地表明，∆NS1 衍生的纳米颗粒（NP-∆NS1）组装成具有高度多聚化的三维结构。虽然 ∆NS1 被证明是一种弱免疫原，但用 NP-∆NS1 免疫增强了亚单位疫苗的免疫原性，使接种小鼠的抗体持续时间延长。因此，用自组装抗原（纳米疫苗）进行免疫代表了一种新的有前途的策略，可基于纯化的重组蛋白增强 NS1 特异性抗体的诱导。