Li Chuan-Jian, Ye Min-An, Su Pei-Pei, Yao Cheng, Zhou Yi
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119153. doi: 10.1016/j.saa.2020.119153. Epub 2020 Nov 4.
Although being as an important chemical material in industry, hydrazine (NH) is highly toxic to the humans and animals. The development of sensitive methods for the detection of hydrazine is meaningful. Herein, we develop a new organic-inorganic hybrid nanoprobe for the detection of NH based on luminescent resonance energy transfer (LRET) process. The nanoprobe contains NH-responsive NIR cyanine dye (CQM1) and α-cyclodextrin (CD) anchored on the surface of lanthanide-doped upconversion nanophosphors (UCNPs). In the presence of hydrazine, the hybrid materials (CQM1-UCNPs) showed the a large ratiometric luminescent signal change with high sensitivity and selectivity. More importantly, by taking advantage of ratiometric Upconversion luminescent (UCL) signal and the features of NIR emission/excitation, the nanoprobe was successfully applied for visualization of hydrazine in living cells for the first time.
尽管肼（NH）作为一种重要的工业化学原料，但对人类和动物具有高毒性。开发检测肼的灵敏方法具有重要意义。在此，我们基于发光共振能量转移（LRET）过程开发了一种用于检测NH的新型有机-无机杂化纳米探针。该纳米探针包含对NH有响应的近红外花菁染料（CQM1）和锚定在镧系掺杂上转换纳米磷光体（UCNPs）表面的α-环糊精（CD）。在肼存在的情况下，杂化材料（CQM1-UCNPs）表现出大的比率发光信号变化，具有高灵敏度和选择性。更重要的是，利用比率上转换发光（UCL）信号和近红外发射/激发的特性，该纳米探针首次成功应用于活细胞中肼的可视化。
