School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Institute of Advanced Materials (IAM) and Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
Biomaterials. 2015 Jun;54:34-43. doi: 10.1016/j.biomaterials.2015.03.003. Epub 2015 Mar 29.
Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.
内源性过氧化氢是与细胞信号转导和内稳态相关的重要参数。然而，活系统中异常的 H2O2 调节与许多病理状况有关。在活系统中监测这种信号是至关重要的，但具有挑战性，因为由于激发源为紫外/可见光，当前的 H2O2 探针对于高效的生物成像来说并不实用。在此，我们提出了一种基于有机荧光团连接的镧系掺杂上转换纳米探针（CYD1-UCNPs）的新型设计，用于选择性 UCL 检测 H2O2。这种纳米探针代表了下一代成像工具，它具有对 H2O2 的鲁棒 UCL“开启”响应，具有 NIR 激发的比率信号，并且在活细胞和整个动物内源性 H2O2 产生的比率 UCL 成像中具有潜在的应用。
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