Han Qingzhi, Wang Hanyu, Wu Dan, Wei Qin
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, China.
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Biosens Bioelectron. 2021 Feb 1;173:112803. doi: 10.1016/j.bios.2020.112803. Epub 2020 Nov 7.
On the basis of synthesized PbS nanoparticles (PbS NPs)/reduced graphene oxide (RGO)/NiO nanosheet arrays (NiO NSAs) heterostructure, we constructed a function-switchable self-powered PEC sensing platform for the analysis of HO and glucose. Ordered NiO NSAs have high electron mobility, modifying RGO onto the surfaces of NiO NSAs can connect the NiO NSAs with the PbS NPs and promoted the electron transfer rate between them, as well as enhance their photocurrent response. The PbS NPs/RGO/NiO NSAs heterostructure own excellent catalase-like activity can achieve HO detection, only with one more step, after introducing glucose oxidase (GOD) onto the surface of PbS NPs/RGO/NiO NSAs heterostructure, we realized the detection conversion between HO and glucose. Under optimal conditions, the proposed biosensor exhibited superior analytical performance toward HO and glucose, a limit of detection (LOD) of 0.018 mM (S/N = 3) and 5.3 × 10 M (S/N = 3) were obtained, respectively. Moreover, good accuracy was obtained in the real samples analysis of HO disinfectant and human serum samples.
基于合成的硫化铅纳米颗粒（PbS NPs）/还原氧化石墨烯（RGO）/氧化镍纳米片阵列（NiO NSAs）异质结构，我们构建了一个功能可切换的自供电光电化学（PEC）传感平台，用于分析过氧化氢（HO）和葡萄糖。有序的NiO NSAs具有高电子迁移率，在NiO NSAs表面修饰RGO可以将NiO NSAs与PbS NPs连接起来，提高它们之间的电子转移速率，并增强其光电流响应。具有优异类过氧化氢酶活性的PbS NPs/RGO/NiO NSAs异质结构能够实现对HO的检测，只需再多一步，在PbS NPs/RGO/NiO NSAs异质结构表面引入葡萄糖氧化酶（GOD）后，我们实现了HO和葡萄糖之间的检测转换。在最佳条件下，所提出的生物传感器对HO和葡萄糖表现出优异的分析性能，分别获得了0.018 mM（S/N = 3）和5.3×10 M（S/N = 3）的检测限（LOD）。此外，在HO消毒剂和人血清样品的实际样品分析中获得了良好的准确度。
