Mesoporous MnFeO magnetic nanoparticles as a peroxidase mimic for the colorimetric detection of urine glucose.

Mesoporous MnFeO magnetic nanoparticles (mMnFeO MNPs) were prepared with a one-step synthesis method and characterized to possess intrinsic peroxidase-like activity, and had obvious advantages over other peroxidase nanozymes in terms of high catalytic affinity, high stability, mono-dispersion, easy preparation, and quick separation. The mMnFeO MNPs were used as a colorimetric sensor for indirect sensing of urine glucose based on the sensing principle that HO can be produced from glucose oxidation catalyzed by glucose oxidase (GOx), and under the catalysis of the mMnFeO MNPs nanozyme, HO can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue color in a few minutes. This sensor is simple, cheap, sensitive, and specific to glucose detection with a detection limit of 0.7 μM, suggesting its potential for on-site glucose detection.