Liu Ke, Su Jiaxing, Liang Jiangong, Wu Yuan
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University Wuhan 430070 China
RSC Adv. 2021 Aug 23;11(45):28375-28380. doi: 10.1039/d1ra05396e. eCollection 2021 Aug 16.
Mesoporous MnFeO magnetic nanoparticles (mMnFeO MNPs) were prepared with a one-step synthesis method and characterized to possess intrinsic peroxidase-like activity, and had obvious advantages over other peroxidase nanozymes in terms of high catalytic affinity, high stability, mono-dispersion, easy preparation, and quick separation. The mMnFeO MNPs were used as a colorimetric sensor for indirect sensing of urine glucose based on the sensing principle that HO can be produced from glucose oxidation catalyzed by glucose oxidase (GOx), and under the catalysis of the mMnFeO MNPs nanozyme, HO can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue color in a few minutes. This sensor is simple, cheap, sensitive, and specific to glucose detection with a detection limit of 0.7 μM, suggesting its potential for on-site glucose detection.
采用一步合成法制备了介孔MnFeO磁性纳米颗粒（mMnFeO MNPs），经表征其具有内在的类过氧化物酶活性，与其他过氧化物酶纳米酶相比，在催化亲和力高、稳定性高、单分散性好、易于制备和快速分离等方面具有明显优势。基于葡萄糖氧化酶（GOx）催化葡萄糖氧化可产生H₂O₂，在mMnFeO MNPs纳米酶的催化下，H₂O₂可在几分钟内将3,3',5,5'-四甲基联苯胺（TMB）氧化产生蓝色的传感原理，mMnFeO MNPs被用作比色传感器用于间接检测尿糖。该传感器简单、廉价、灵敏且对葡萄糖检测具有特异性，检测限为0.7 μM，表明其具有现场检测葡萄糖的潜力。
