el Asrar A M, Geboes K, Missotten L, Emarah M H, Maudgal P C, Desmet V
Department of Ophthalmology, Mansoura University Hospital, Egypt.
Br J Ophthalmol. 1987 Nov;71(11):867-72. doi: 10.1136/bjo.71.11.867.
The cellular composition of the inflammatory infiltrate present in 13 patients with the limbal form of vernal keratoconjunctivitis was examined in the conjunctival scrapings and the limbic replicas by means of Giemsa stain and immunohistochemistry. Conjunctival scrapings showed the presence of mast cells, lymphocytes, plasma cells, polymorphonuclear leucocytes, and very few basophils in all the specimens. Eosinophils were present in only four scrapings. The superficial epithelium of the limbic lesion and the adjacent cornea and conjunctiva was studied by the replica technique. The limbic lesion area showed the presence of necrotic epithelial cells mixed with inflammatory cells, including eosinophils, mast cells, lymphocytes, plasma cells, and polymorphonuclear leucocytes and very few basophils. Most of the inflammatory cells were HLA-DR+. Many OKT6+ cells were present, indicating the presence of Langerhans cells. T-lymphocytes including a few helper/inducer cells and many suppressor/cytotoxic cells, were detected in the infiltrate. In addition many B-lymphocytes were observed. These findings suggest that other immune mechanisms in addition to type 1 reaction are involved in the pathogenesis of the disease.
通过吉姆萨染色和免疫组织化学方法，对13例睑裂部春季角结膜炎患者结膜刮片和睑裂部印片的炎症浸润细胞成分进行了检查。所有标本的结膜刮片均显示有肥大细胞、淋巴细胞、浆细胞、多形核白细胞，以及极少数嗜碱性粒细胞。仅4例刮片中发现有嗜酸性粒细胞。采用印片技术对睑裂部病变及相邻角膜和结膜的表层上皮进行了研究。睑裂部病变区域可见坏死上皮细胞与炎症细胞混合存在，炎症细胞包括嗜酸性粒细胞、肥大细胞、淋巴细胞、浆细胞、多形核白细胞和极少数嗜碱性粒细胞。大多数炎症细胞HLA - DR阳性。存在许多OKT6阳性细胞，表明有朗格汉斯细胞。浸润中检测到T淋巴细胞，包括少数辅助/诱导细胞和许多抑制/细胞毒性细胞。此外，还观察到许多B淋巴细胞。这些发现提示，除1型反应外，其他免疫机制也参与了该病的发病过程。
