从牛肺中分离并鉴定一种与胶原纤维相关的硫酸皮肤素蛋白聚糖。

Isolation and characterization of a collagen fibril-associated dermatan sulphate proteoglycan from bovine lung.

作者信息

van Kuppevelt T H, Janssen H M, van Beuningen H M, Cheung K S, Schijen M M, Kuyper C M, Veerkamp J H

机构信息

Department of Chemical Cytology, University of Nijmegen, The Netherlands.

出版信息

Biochim Biophys Acta. 1987 Dec 7;926(3):296-309. doi: 10.1016/0304-4165(87)90216-9.

DOI:10.1016/0304-4165(87)90216-9

PMID:3318935

Abstract

Dermatan sulphate proteoglycans have been extracted from bovine lung with 2.0 M CaCl2 and isolated using CsCl density gradient centrifugation, DEAE ion-exchange chromatography, gel chromatography and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Ultrastructurally these proteoglycans are specifically associated with collagen fibrils. Dermatan sulphate (Mr 15.10(3)-35.10(3), with a strong prevalence for the higher Mr) is link via an O-glycosidic bond to a protein core, which is rich in Asx, Glx and Leu. Of the total uronic acid, 91% is iduronic acid. A part of the glucuronic acid residues is located near the protein core and a large cluster of disaccharides is devoid of glucuronic acid residues. An inhibition enzyme immunoassay has been developed to quantitate the proteoglycan. A model for the interaction between dermatan sulphate proteoglycans and collagen fibrils is proposed.

摘要

硫酸皮肤素蛋白聚糖已用2.0M氯化钙从牛肺中提取出来，并通过氯化铯密度梯度离心、DEAE离子交换色谱、凝胶色谱和制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离。从超微结构来看，这些蛋白聚糖与胶原纤维特异性相关。硫酸皮肤素（相对分子质量为15.10（3）-35.10（3），以较高相对分子质量者为主）通过O-糖苷键与富含天冬氨酸、谷氨酸和亮氨酸的蛋白质核心相连。在总糖醛酸中，91%为艾杜糖醛酸。部分葡萄糖醛酸残基位于蛋白质核心附近，一大簇二糖不含葡萄糖醛酸残基。已开发出一种抑制酶免疫测定法来定量该蛋白聚糖。本文提出了硫酸皮肤素蛋白聚糖与胶原纤维之间相互作用的模型。