Uldbjerg N, Malmström A, Ekman G, Sheehan J, Ulmsten U, Wingerup L
Biochem J. 1983 Feb 1;209(2):497-503. doi: 10.1042/bj2090497.
Proteoglycans were extracted from human uterine cervix with 4 M-guanidinium chloride in the presence of proteinase inhibitors. They were purified by density-gradient centrifugation in 4 M-guanidinium chloride/CsCl (starting density 1.32 g/ml) followed by DEAE-cellulose and Sepharose chromatography. Only one polydisperse proteoglycan was found. s020,w was 2.1S and the weight-average molecular weight was 73 000 (sedimentation-equilibrium centrifugation) to 110 500 (light-scattering). The core protein was monodisperse, with an apparent molecular weight of 47 000. The proteoglycan contained about 30% protein and probably two or three glycosaminoglycan side chains per molecule. High contents of aspartate, glutamate and leucine were found. The glycan moiety of the proteoglycan was exclusively dermatan sulphate, with a co-polymeric structure with approximately equal quantities of iduronic acid- and glucuronic acid-containing disaccharides.
在蛋白酶抑制剂存在的情况下，用4M氯化胍从人子宫颈中提取蛋白聚糖。通过在4M氯化胍/ CsCl（起始密度1.32g / ml）中进行密度梯度离心，然后进行DEAE - 纤维素和琼脂糖凝胶色谱法进行纯化。仅发现一种多分散蛋白聚糖。s020，w为2.1S，重均分子量为73000（沉降平衡离心法）至110500（光散射法）。核心蛋白是单分散的，表观分子量为47000。该蛋白聚糖每分子含有约30％的蛋白质，可能有两到三条糖胺聚糖侧链。发现天冬氨酸、谷氨酸和亮氨酸的含量很高。该蛋白聚糖的聚糖部分仅为硫酸皮肤素，具有含艾杜糖醛酸和葡萄糖醛酸的二糖数量大致相等的共聚结构。
