含磷酸钙纳米粒子的可再充电黏合剂可抑制生物膜挑战环境下的牙本质长期脱矿。
Rechargeable adhesive with calcium phosphate nanoparticles inhibited long-term dentin demineralization in a biofilm-challenged environment.
机构信息
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
出版信息
J Dent. 2021 Jan;104:103529. doi: 10.1016/j.jdent.2020.103529. Epub 2020 Nov 12.
OBJECTIVES
This study aims to investigate the long-term demineralization-inhibition capability of a rechargeable adhesive with nanoparticles of amorphous calcium phosphate (NACP) on dentin in a biofilm-challenged environment.
METHODS
The NACP adhesive was immersed in a pH 4 solution to exhaust calcium (Ca) and phosphate (P) ions and then recharged with Ca and P ions. Dentin samples were demineralized underStreptococcus mutans biofilms for 24 h and randomly divided into two groups: (1) dentin control, (2) dentin with recharged NACP adhesives. Each day, all the samples were immersed in brain heart infusion broth with 1% sucrose (BHIS) for 4 h, and then in artificial saliva (AS) for 20 h. This cycle was repeated for 10 days. The pH of BHIS, the Ca and P ions content of the BHIS and AS were measured daily. After 10 days, the lactic acid production and colony-forming units of the biofilms were tested. The changes of remineralization/demineralization were also analyzed.
RESULTS
Dentin in the control group showed further demineralization. The recharged NACP adhesive neutralized acids, increasing the pH to above 5, and released large amounts of Ca and P ions each day. The recharged NACP adhesive decreased the production of lactic acid (P < 0.05), inhibited dentin demineralization and sustained the dentin hardness in the biofilm-challenged environment, showing an excellent long-term demineralization-inhibition capability.
CONCLUSIONS
The NACP adhesive could continuously inhibit dentin demineralization in a biofilm-challenged environment by recharging with Ca and P ions.
SIGNIFICANCE
The rechargeable NACP adhesive could provide long-term dentin bond protection.
目的
本研究旨在探究纳米磷酸钙（NACP）可再充电胶在生物膜挑战环境下对牙本质的长期脱矿抑制能力。
方法
将 NACP 胶浸泡在 pH 4 的溶液中，以耗尽钙（Ca）和磷酸盐（P）离子，然后再用 Ca 和 P 离子进行再充电。将牙本质样本在变形链球菌生物膜下脱矿 24 h，并随机分为两组：（1）牙本质对照组，（2）用再充电 NACP 胶处理的牙本质。每天，所有样本均在含有 1%蔗糖的脑心浸液肉汤（BHIS）中浸泡 4 h，然后在人工唾液（AS）中浸泡 20 h。该循环重复 10 天。每天测量 BHIS 的 pH、BHIS 和 AS 中的 Ca 和 P 离子含量。10 天后，测试生物膜的乳酸产量和菌落形成单位。还分析了再矿化/脱矿质的变化。
结果
对照组牙本质进一步脱矿。再充电的 NACP 胶中和酸，将 pH 值提高到 5 以上，并每天释放大量的 Ca 和 P 离子。再充电的 NACP 胶减少了乳酸的产生（P<0.05），抑制了牙本质脱矿，并在生物膜挑战环境中维持了牙本质硬度，表现出优异的长期脱矿抑制能力。
结论
NACP 胶可通过再充电 Ca 和 P 离子持续抑制生物膜挑战环境下的牙本质脱矿。
意义
可再充电的 NACP 胶可为牙本质提供长期的粘结保护。
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