Al-Qarni Faisal, Weir Michael, Melo Mary A, Al-Dulaijan Yousif, Almulhim Khalid S, Xu Hockin H K
Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA.
Clin Oral Investig. 2022 Jan;26(1):313-323. doi: 10.1007/s00784-021-04002-7. Epub 2021 Jun 10.
This study aimed to develop an antibacterial and calcium (Ca) and phosphate (P) rechargeable adhesive and investigate the effects of dimethylaminododecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) on dentin bonding, biofilm response, and repeated Ca and P ion recharge and re-release capability for the first time.
Pyromellitic glycerol dimethacrylate (PMGDM), ethoxylated bisphenol A dimethacrylate (EBPADMA), 2-hydroxyethyl methacrylate (HEMA), and bisphenol A glycidyl dimethacrylate (BisGMA) formed the adhesive (PEHB). Three groups were tested: (1) Scotchbond (SBMP, 3 M) control, (2) PEHB + 30% NACP, and (3) PEHB + 30% NACP + 5% DMAHDM. Specimens were tested for dentin shear bond strength, and Ca and P ion release, recharge, and re-release. Biofilm lactic acid production and colony-forming units (CFU) on resins were analyzed.
The four groups had similar dentin shear bond strengths (p > 0.1). Adhesive with DMAHDM showed significant decrease in metabolic activity, lactic acid production, and biofilm CFU (p < 0.05). The adhesives containing NACP released high levels of Ca and P ions initially and after being recharged.
This study developed the first Ca and P ion-rechargeable and antibacterial adhesive, achieving strong antibacterial activity and Ca and P ion recharge and re-release for long-term remineralization.
Considering the restoration-tooth bonded interface being the weak link and recurrent caries at the margins being the primary reason for restoration failures, this novel calcium phosphate-rechargeable and antibacterial adhesive is promising for a wide range of tooth-restoration applications to inhibit caries.
本研究旨在开发一种抗菌且可再充钙（Ca）和磷（P）的粘合剂，并首次研究甲基丙烯酸二甲氨基十二烷基酯（DMAHDM）和无定形磷酸钙纳米颗粒（NACP）对牙本质粘结、生物膜反应以及重复的Ca和P离子再充注和再释放能力的影响。
均苯四甲酸甘油二甲基丙烯酸酯（PMGDM）、乙氧基化双酚A二甲基丙烯酸酯（EBPADMA）、甲基丙烯酸2-羟乙酯（HEMA）和双酚A缩水甘油二甲基丙烯酸酯（BisGMA）构成粘合剂（PEHB）。测试了三组：（1）Scotchbond（SBMP，3M）对照组，（2）PEHB + 30% NACP，以及（3）PEHB + 30% NACP + 5% DMAHDM。对样本进行牙本质剪切粘结强度以及Ca和P离子释放、再充注和再释放测试。分析了树脂上生物膜乳酸产量和菌落形成单位（CFU）。
四组的牙本质剪切粘结强度相似（p > 0.1）。含有DMAHDM的粘合剂在代谢活性、乳酸产量和生物膜CFU方面显著降低（p < 0.05）。含NACP的粘合剂在初始时以及再充注后释放高水平的Ca和P离子。
本研究开发出了首款可再充Ca和P离子的抗菌粘合剂，实现了强大的抗菌活性以及Ca和P离子的再充注和再释放，以实现长期再矿化。
鉴于修复体与牙齿的粘结界面是薄弱环节，边缘复发性龋齿是修复失败的主要原因，这种新型的可再充磷酸钙抗菌粘合剂在广泛的牙齿修复应用中有望抑制龋齿。
