分析痰/气管吸出物和鼻咽样本，使用实验室开发的检测方法和 Panther Fusion 系统检测 SARS-CoV-2。
Analysis of sputum/tracheal aspirate and nasopharyngeal samples for SARS-CoV-2 detection by laboratory-developed test and Panther Fusion system.
机构信息
Department of Pathology, University of Texas Medical Branch, Galveston, TX.
Department of Pathology, University of Texas Medical Branch, Galveston, TX.
出版信息
Diagn Microbiol Infect Dis. 2021 Jan;99(1):115228. doi: 10.1016/j.diagmicrobio.2020.115228. Epub 2020 Sep 30.
Abstract
In this study, 127 sputum/tracheal aspirate specimens were evaluated by a laboratory-developed real-time RT-PCR method and Fusion SARS-CoV-2 assay. These specimens were collected from the patients who have nasopharyngeal swab (NPS) samples being used for SARS-CoV-2 detection previously or simultaneously. The overall agreement was 96% between the lower respiratory tract (LRT) and NPS samples, suggesting that LRT specimens could be an option for patients who develop a productive cough or those receiving invasive mechanical ventilation.
摘要
本研究采用实验室开发的实时 RT-PCR 方法和 Fusion SARS-CoV-2 检测试剂盒对 127 份痰/气管吸出物标本进行了评估。这些标本是从先前或同时采集了用于 SARS-CoV-2 检测的鼻咽拭子（NPS）样本的患者中收集的。下呼吸道（LRT）样本与 NPS 样本之间的总体一致性为 96%，这表明对于出现有痰咳嗽或接受有创机械通气的患者，LRT 标本可能是一种选择。
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本文引用的文献
1
SARS-CoV-2 detection in different respiratory sites: A systematic review and meta-analysis.不同呼吸道部位检测 SARS-CoV-2：系统评价和荟萃分析。
EBioMedicine. 2020 Sep;59:102903. doi: 10.1016/j.ebiom.2020.102903. Epub 2020 Jul 24.
2
Differences of Severe Acute Respiratory Syndrome Coronavirus 2 Shedding Duration in Sputum and Nasopharyngeal Swab Specimens Among Adult Inpatients With Coronavirus Disease 2019.新型冠状病毒肺炎成年住院患者痰液与鼻咽拭子标本中 2019 年冠状病毒脱落持续时间的差异。
Chest. 2020 Nov;158(5):1876-1884. doi: 10.1016/j.chest.2020.06.015. Epub 2020 Jun 20.
3
Virological assessment of hospitalized patients with COVID-2019.住院 COVID-19 患者的病毒学评估。
Nature. 2020 May;581(7809):465-469. doi: 10.1038/s41586-020-2196-x. Epub 2020 Apr 1.
4
SARS-CoV-2-Positive Sputum and Feces After Conversion of Pharyngeal Samples in Patients With COVID-19.新型冠状病毒肺炎患者咽拭子转阴后痰液和粪便中仍存在严重急性呼吸综合征冠状病毒2阳性
Ann Intern Med. 2020 Jun 16;172(12):832-834. doi: 10.7326/M20-0991. Epub 2020 Mar 30.
5
Detection of SARS-CoV-2 in Different Types of Clinical Specimens.SARS-CoV-2 在不同类型临床标本中的检测。
JAMA. 2020 May 12;323(18):1843-1844. doi: 10.1001/jama.2020.3786.
6
Detection of respiratory viruses in sputum from adults by use of automated multiplex PCR.利用自动化多重聚合酶链反应检测成人痰液中的呼吸道病毒。
J Clin Microbiol. 2014 Oct;52(10):3590-6. doi: 10.1128/JCM.01523-14. Epub 2014 Jul 23.
7
The measurement of observer agreement for categorical data.分类数据观察者一致性的测量。
Biometrics. 1977 Mar;33(1):159-74.
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