Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120, Heidelberg, Germany.
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120, Heidelberg, Germany.
Curr Opin Cell Biol. 2021 Feb;68:124-131. doi: 10.1016/j.ceb.2020.10.004. Epub 2020 Nov 12.
Microtubules are essential cytoskeletal elements assembled from αβ-tubulin dimers. In high eukaryotes, microtubule nucleation, the de novo assembly of a microtubule from its minus end, is initiated by the γ-tubulin ring complex (γ-TuRC). Despite many years of research, the structural and mechanistic principles of the microtubule nucleation machinery remained poorly understood. Only recently, cryoelectron microscopy studies uncovered the molecular organization and potential activation mechanisms of γ-TuRC. In vitro assays further deciphered the spatial and temporal cooperation between γ-TuRC and additional factors, for example, the augmin complex, the phase separation protein TPX2, and the microtubule polymerase XMAP215. These breakthroughs deepen our understanding of microtubule nucleation mechanisms and will link the assembly of individual microtubules to the organization of cellular microtubule networks.
微管是由αβ-微管二聚体组装而成的重要细胞骨架元件。在高等真核生物中，微管的成核作用，即从微管的负端从头组装微管，是由γ-微管蛋白环复合物（γ-TuRC）启动的。尽管经过多年的研究，但微管成核机制的结构和机制原理仍知之甚少。直到最近，冷冻电子显微镜研究才揭示了 γ-TuRC 的分子组织和潜在的激活机制。体外实验进一步解析了 γ-TuRC 与其他因素（例如，augmin 复合物、相分离蛋白 TPX2 和微管聚合酶 XMAP215）之间的空间和时间合作。这些突破加深了我们对微管成核机制的理解，并将单个微管的组装与细胞微管网络的组织联系起来。
