冲击波应用增强了牙本质脱敏剂的效果。

Shockwave application enhances the effect of dentin desensitizer.

机构信息

Department of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.

Forschungszentrum Juelich, ICS-3, Soft Matter, Juelich, Germany.

出版信息

Dent Mater. 2021 Jan;37(1):113-119. doi: 10.1016/j.dental.2020.10.027. Epub 2020 Nov 13.

DOI:10.1016/j.dental.2020.10.027

PMID:33190860

Abstract

OBJECTIVES

The purpose of this study was to develop a new device that can improve the effect of desensitizer using shockwaves and to verify its efficacy.

METHODS

A micro-shockwave generator was developed using a piezoelectric actuator (PIA-1000, piezosystem jena GmbH, Jena, Germany), an Arduino Uno microcontroller (Arduino, Torino, Italy), and a high voltage pulser (HVP-1000, piezosystem jena GmbH) at 700 V (400 A) and 100 μs. The occlusal surfaces of 20 extracted human upper and lower third molars without caries or restoration were reduced to expose the occlusal dentin, and the prepared occlusal surfaces were acid-etched with 32% phosphoric acid to remove the smear layer. The tooth specimens were connected to a fluid flow measurement instrument (nanoFlow, IB SYSTEMS, Seoul, Korea), permeability through dentin via dentinal fluid flow (DFF) was measured for 300 s, and the average DFF rate (Baseline DFF rate) was calculated. A desensitizer (SuperSeal, Phoenix Dental, Fenton, MI, USA) was applied to the acid-etched occlusal dentin surface of 10 randomly selected tooth specimens, left for 10 s, and rubbed with a microbrush for 30 s (Group 1). For the remaining teeth, the desensitizer was applied, and a shockwave (100 μm stroke, 10,000 G) was applied for 10 s (2 shots/s) and rubbed with a microbrush for 30 s (Group 2). After desensitizer application, subsequent DFF was measured for 600 s, and the average DFF rate was calculated (post-application DFF rate). DFF was continuously measured in real-time at 25 ± 0.5 ℃ under a hydrostatic pressure of 25 cm. The percentage reduction in DFF rate after desensitizer application (with or without shockwave) was calculated with respect to baseline DFF rate. Data were analyzed with independent t-test (α = 0.05).

RESULTS

For all tooth specimens, DFF rate decreased after desensitizer application irrespective of the presence of shockwaves. The percentage reduction in DFF rate of SuperSeal with shockwave (Group 2) was 42.8 ± 19.0%, which was significantly higher than the 26.2 ± 13.6% of the SuperSeal only group (Group 1) (p < 0.05).

SIGNIFICANCE

Measurement of DFF change in real-time shows that shockwaves can help reduce dentin permeability beyond that SuperSeal dentin desensitizer produced alone.

摘要

目的

本研究旨在开发一种使用冲击波增强脱敏剂效果的新设备，并验证其疗效。

方法

使用压电致动器（PIA-1000，德国耶拿 piezo system jena 公司）、Arduino Uno 微控制器（意大利都灵 Arduino）和高压脉冲器（HVP-1000，德国耶拿 piezo system jena 公司）在 700V（400A）和 100μs 下开发了微冲击波发生器。将 20 个无龋或修复的上颌和下颌第三磨牙的咬合面磨至暴露牙本质，并用 32%磷酸酸蚀剂处理牙本质表面以去除玷污层。将牙本质标本连接到流体流量测量仪器（nanoFlow，韩国 IB SYSTEMS）上，通过牙本质流体流动测量牙本质通透性（DFF），持续 300s，计算平均 DFF 率（基线 DFF 率）。将脱敏剂（SuperSeal，美国菲尼克斯牙科 Phoenix Dental）应用于 10 个随机选择的牙本质标本的酸蚀牙合面，保持 10s，并用微型牙刷摩擦 30s（第 1 组）。对于其余牙齿，应用脱敏剂，应用冲击波（100μm 行程，10000G）10s（2 次/秒）并用微型牙刷摩擦 30s（第 2 组）。应用脱敏剂后，连续测量 600s 的后续 DFF，并计算平均 DFF 率（应用后 DFF 率）。在 25±0.5℃的恒水压下，实时连续测量 DFF。以基线 DFF 率为基准，计算应用脱敏剂（有或无冲击波）后 DFF 率的降低百分比。采用独立 t 检验（α=0.05）进行数据分析。