College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China; Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Environ Pollut. 2021 Mar 1;272:116013. doi: 10.1016/j.envpol.2020.116013. Epub 2020 Nov 6.
In this study, functional microbial sequencing, quantitative PCR, and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were employed to understand the microbial mechanisms related to the effects of bamboo charcoal (BC) and bamboo vinegar (BV) on the degradation of organic matter (OM) and methane (CH) emissions during composting. BC + BV resulted in the highest degradation of OM. BV was most effective treatment in controlling CH emissions and it significantly reduced the abundance of the mcrA gene. Methanobrevibacter, Methanosarcina, and Methanocorpusculum were closely related to CH emissions during the thermophilic composting period. PICRUSt analysis showed that BC and/or BV enhanced the metabolism associated with OM degradation and reduced CH metabolism. Structural equation modeling indicated that BC + BV strongly promoted the metabolic activity of microorganisms, which had a positive effect on CH emissions. Together these results suggest that BC + BV may be a suitable composting strategy if the aerobic conditions can be effectively improved during the thermophilic composting period.
在这项研究中，采用功能微生物测序、定量 PCR 和未观察状态重建的群落系统发育分析（PICRUSt），以了解竹炭（BC）和竹醋液（BV）对堆肥中有机物（OM）降解和甲烷（CH）排放影响的微生物机制。BC+BV 导致 OM 降解效果最佳。BV 是控制 CH 排放最有效的处理方法，它显著降低了 mcrA 基因的丰度。在高温堆肥期间，甲烷杆菌属、甲烷八叠球菌属和甲烷微菌属与 CH 排放密切相关。PICRUSt 分析表明，BC 和/或 BV 增强了与 OM 降解相关的代谢作用，降低了 CH 代谢。结构方程模型表明，BC+BV 强烈促进了微生物的代谢活性，对 CH 排放有积极影响。综上所述，如果能在高温堆肥期间有效改善好氧条件，BC+BV 可能是一种合适的堆肥策略。
