Wen Ping, Tang Jia, Wang Yueqiang, Liu Xiaoming, Yu Zhen, Zhou Shungui
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
Sci Total Environ. 2021 Aug 25;784:147179. doi: 10.1016/j.scitotenv.2021.147179. Epub 2021 Apr 18.
Methane (CH) emissions from thermophilic composting (TC) are a substantial contributor to climate change. Hyperthermophilic composting (HTC) can influence CH-related microbial communities at temperatures up to 80 °C, and thus impact the CH emissions during composting. This work investigated CH emissions in sludge-derived HTC, and explored microbial community succession with quantitative PCR and high-throughput sequencing. Results demonstrated that HTC decreased CH emissions by 52.5% compared with TC. In HTC, the CH production potential and CH oxidation potential were nearly 40% and 64.1% lower than that of TC, respectively. There was a reduction in the quantity of mcrA (3.7 × 10 to 0 g TS) in HTC, which was more significant than the reduction in pmoA (2.0 × 10 to 2.1 × 10 g TS), and thus lead to reduce CH emissions. It was found that the abundance of most methanogens and methanotrophs was inhibited in the hyperthermal environment, with a decline in Methanosarcina, Methanosaeta and Methanobrevibacter potentially being responsible for reducing the CH emissions in HTC. This work provides important insight into mitigating CH emissions in composting.
嗜热堆肥（TC）产生的甲烷（CH）排放是气候变化的重要促成因素。超高温堆肥（HTC）在高达80°C的温度下会影响与CH相关的微生物群落，从而影响堆肥过程中的CH排放。本研究调查了污泥衍生的HTC中的CH排放情况，并通过定量PCR和高通量测序探索了微生物群落演替。结果表明，与TC相比，HTC使CH排放量降低了52.5%。在HTC中，CH产生潜力和CH氧化潜力分别比TC低近40%和64.1%。HTC中mcrA的数量减少（从3.7×10到0 g TS），这比pmoA的减少（从2.0×10到2.1×10 g TS）更显著，从而导致CH排放减少。研究发现，在超高温环境中，大多数产甲烷菌和甲烷氧化菌的丰度受到抑制，甲烷八叠球菌、甲烷丝状菌和甲烷短杆菌数量的下降可能是HTC中CH排放减少的原因。这项工作为减轻堆肥过程中的CH排放提供了重要见解。
