强直性脊柱炎血清学诊断的研究尝试。

Attempts towards a serological diagnosis of ankylosing spondylitis.

作者信息

Lakomek H J, Schwochau M, Decken K, Juli E, Will H, Krüskemper H L

机构信息

Department of Medicine C, University of Düsseldorf, West Germany.

出版信息

Clin Rheumatol. 1987 Sep;6 Suppl 2:67-72. doi: 10.1007/BF02203387.

DOI:10.1007/BF02203387

PMID:3319358

Abstract

Polytene chromosomes of salivary glands as well as nuclear proteins from Kc-cells of Drosophila melanogaster have been used as substrate to identify and evaluate the diagnostic value of crossreacting antibodies present in sera of AS patients. The diagnostic significance of the recently described anti-93D antibody (Lakomek et al., 1984) was confirmed by screening sera of patients with definite or suspected AS using cytoimmunofluorescence on the polytene chromosomes. In addition, four new antibodies could be identified in AS sera by immunoblotting. Simultaneous detection of these antibodies supports the diagnosis of AS and is most useful in diagnosis of early stages of this disease.

摘要

果蝇唾液腺的多线染色体以及黑腹果蝇Kc细胞的核蛋白已被用作底物，以鉴定和评估强直性脊柱炎（AS）患者血清中存在的交叉反应抗体的诊断价值。通过对多线染色体进行细胞免疫荧光检测，筛查确诊或疑似AS患者的血清，证实了最近描述的抗93D抗体（Lakomek等人，1984年）的诊断意义。此外，通过免疫印迹法在AS患者血清中鉴定出四种新抗体。同时检测这些抗体有助于AS的诊断，对该病早期阶段的诊断最为有用。