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果蝇黑腹果蝇的热休克胀泡93D：一种与可能具有储存功能的大颗粒相关的核糖核蛋白特异性抗原的积累。

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

作者信息

Dangli A, Grond C, Kloetzel P, Bautz E K

出版信息

EMBO J. 1983;2(10):1747-51. doi: 10.1002/j.1460-2075.1983.tb01652.x.

DOI:10.1002/j.1460-2075.1983.tb01652.x
PMID:6416825
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC555353/
Abstract

The monoclonal antibody P11 is directed against a 38 000 dalton protein of Drosophila melanogaster. On polytene chromosomes this protein is present in a subset of the RNA polymerase II-containing loci. Here we show by density centrifugation and enzyme-linked immunosorbent assay tests that the P11 antigen is part of nuclear ribonucleoprotein (RNP) complexes. Indirect immunofluorescence shows that, after prolonged heat-shock, the P11 antigen is present only in the heat-shock puff 93 D. Identical distribution patterns were obtained with another monoclonal antibody, Q18. Unlike P11, this antibody also cross-reacts with D. hydei and D. virilis polytene chromosomes, where the puffs 48 B and 20 CD, respectively, are the only loci prominently stained after heat-shock. The small and giant RNP complexes previously described in these puffs were also observed in puff 93 D. Both types of particle contain the P11 antigen as shown by immunoelectron microscopy. We suggest that the P11 antigen is associated with a special class of RNPs which are possibly involved in the storage of primary transcription products inside the nucleus.

摘要

单克隆抗体P11针对的是黑腹果蝇的一种38000道尔顿的蛋白质。在多线染色体上，这种蛋白质存在于含RNA聚合酶II的基因座的一个子集中。在这里，我们通过密度离心和酶联免疫吸附试验表明，P11抗原是核糖核蛋白（RNP）复合物的一部分。间接免疫荧光显示，长时间热休克后，P11抗原仅存在于热休克胀泡93D中。用另一种单克隆抗体Q18也获得了相同的分布模式。与P11不同，这种抗体还能与海德氏果蝇和粗壮果蝇的多线染色体发生交叉反应，热休克后，分别只有胀泡48B和20CD这两个基因座被显著染色。在胀泡93D中也观察到了先前在这些胀泡中描述的小RNP复合物和大RNP复合物。免疫电子显微镜显示，这两种颗粒都含有P11抗原。我们认为，P11抗原与一类特殊的RNP相关，这类RNP可能参与细胞核内初级转录产物的储存。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/440a4cc436ab/emboj00263-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/bafcc37cfb3c/emboj00263-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/bca52b94e9b0/emboj00263-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/4ff446145223/emboj00263-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/f749d2f48f7f/emboj00263-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/440a4cc436ab/emboj00263-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/bafcc37cfb3c/emboj00263-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/bca52b94e9b0/emboj00263-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/4ff446145223/emboj00263-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/f749d2f48f7f/emboj00263-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2701/555353/440a4cc436ab/emboj00263-0126-a.jpg

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Interaction of the hnRNA of amphibian oocytes with fibril-forming proteins.两栖类卵母细胞的核内不均一RNA与原纤维形成蛋白的相互作用。
Eur J Biochem. 1982 Oct;127(2):301-8. doi: 10.1111/j.1432-1033.1982.tb06870.x.
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Recombination frequency and DNA content of the distal part of the second chromosome of Drosophila hydei Sturtevant.
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Chromosoma. 2015 Sep;124(3):367-83. doi: 10.1007/s00412-015-0506-0. Epub 2015 Feb 8.
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The large noncoding hsrω-n transcripts are essential for thermotolerance and remobilization of hnRNPs, HP1 and RNA polymerase II during recovery from heat shock in Drosophila.大型非编码hsrω-n转录本对于果蝇热休克恢复过程中的耐热性以及hnRNPs、HP1和RNA聚合酶II的重新 mobilization 至关重要。（注：“remobilization”这里不太明确准确的中文对应词，可根据具体医学背景进一步优化表述）
Chromosoma. 2012 Feb;121(1):49-70. doi: 10.1007/s00412-011-0341-x. Epub 2011 Sep 9.
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Forty years of the 93D puff of Drosophila melanogaster.果蝇 93D 连续喷射 40 年。
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Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.果蝇A1-异质性核糖核蛋白同源物的两个RNA结合结构域在体内的可分离作用。
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Rapid sequence divergence in a heat shock locus of Drosophila.果蝇热休克基因座中的快速序列分歧。
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Specific activation of puff 93D of Drosophila melanogaster by benzamide and the effect of benzamide treatment on the heat shock induced puffing activity.苯甲酰胺对黑腹果蝇93D疏松区的特异性激活作用以及苯甲酰胺处理对热休克诱导的疏松区活性的影响。
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