Induction of mitophagy in C2C12 cells by electrical pulse stimulation involves increasing the level of the mitochondrial receptor FUNDC1 through the AMPK-ULK1 pathway.

The mitochondrial receptor protein FUN14 domain-containing-1 (FUNDC1) can induce mitophagy under hypoxic conditions, as well as playing important roles in normal metabolism and intracellular homeostasis. Exercise not only elevates mitochondrial biosynthesis, but also exerts a significant impact on mitochondrial fission, integration and mitophagy. However, it is still not clear whether FUNDC1 plays a regulatory role in this context. Electrical pulse stimulation (EPS) of cultured myotubes is widely used as an model of muscle contraction. We simulated the contraction of C2C12 myotubes by EPS (15 V, 1 Hz, 2 ms, 1 h) to examine the role of FUNDC1 in mitophagy. EPS was found to induce mitophagy by activating the AMPK-ULK1 pathway to an even greater extent than AICAR and FUNDC1 is involved in the associated mitophagy. However, when AMPK is inhibited, other pathways may regulate mitophagy. Our findings indicate that mitophagy helps maintain the normal functions of mitochondria. EPS of C2C12 myotubes results in contraction, induction of mitophagy and potential activation of the AMPK-ULK1 pathway that promotes the expression of FUNDC1.