Research Center of Basic Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
Department of Medical Laboratory, Weifang Medical University, Weifang, China.
J Cell Physiol. 2021 Mar;236(3):1787-1797. doi: 10.1002/jcp.29956. Epub 2020 Jul 28.
Recent studies have shown that the overexpression of α5 nicotinic acetylcholine receptor (α5-nAChR) is associated with nicotine-related lung carcinogenesis. Survivin is one of the biomarkers of a worse prognosis for smoking-related lung cancer. The aim of this study is to investigate the association of α5-nAChR, survivin, and clinical outcomes in lung adenocarcinoma (LUAD). We analyzed the expression level and correlation of CHRNA5 (encoding α5-nAChR) and BIRC5 (encoding survivin) in LUAD with The Cancer Genome Atlas data set. The relationship between overall survival (OS) and the expression of CHRNA5 or/and BIRC5 was evaluated by the Kaplan-Meier method and Cox proportional hazards model. Moreover, our results showed that the expression of α5-nAChR mediated survivin expression in lung cancer cells and in lung tumor xenografts. Relationships between the expression of α5-nAChR and/or survivin with clinical-pathological characteristics were analyzed using LUAD tissue samples. The results showed that expression of α5-nAChR was correlated with survivin expression in vitro and in vivo. The group coexpressing α5-nAChR and survivin had a worse prognosis than other subgroups in LUAD (p < .05). In conclusion, ascertaining the expression of both α5-nAChR and survivin provides a better measure of prognosis for LUAD patients. The combined inhibition of α5-nAChR and survivin may be a promising multitargeted gene therapeutic strategy in LUAD diagnosis.
最近的研究表明，α5 型烟碱型乙酰胆碱受体（α5-nAChR）的过表达与尼古丁相关的肺癌发生有关。Survivin 是与吸烟相关的肺癌预后不良的生物标志物之一。本研究旨在探讨α5-nAChR、Survivin 与肺腺癌（LUAD）临床结局的相关性。我们分析了 CHRNA5（编码α5-nAChR）和 BIRC5（编码 Survivin）在 LUAD 中的表达水平及其相关性与 The Cancer Genome Atlas 数据集。通过 Kaplan-Meier 方法和 Cox 比例风险模型评估 CHRNA5 或/和 BIRC5 表达与总生存期（OS）的关系。此外，我们的结果表明，α5-nAChR 表达在肺癌细胞和肺肿瘤异种移植中调节 Survivin 表达。使用 LUAD 组织样本分析了α5-nAChR 和/或 Survivin 表达与临床病理特征之间的关系。结果表明，α5-nAChR 表达与体外和体内 Survivin 表达相关。在 LUAD 中，共表达α5-nAChR 和 Survivin 的组比其他亚组预后更差（p <.05）。总之，确定α5-nAChR 和 Survivin 的表达提供了 LUAD 患者更好的预后衡量标准。联合抑制α5-nAChR 和 Survivin 可能是 LUAD 诊断中一种有前途的多靶点基因治疗策略。
