Seroogy K B, Mehta A, Fallon J H
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Exp Brain Res. 1987;68(2):277-89. doi: 10.1007/BF00248793.
The colocalization of neurotensin- and cholecystokinin-like immunoreactivities was demonstrated in neurons of the ventral mesencephalon of the rat by using a double-labeling indirect immunofluorescence procedure for the simultaneous detection of two antigens in the same tissue section. Greater than 90% of the neurotensin-positive perikarya distributed throughout the ventral midbrain (primarily located in the ventral tegmental area, medial substantial nigra, and rostral and caudal linear raphe nuclei) were found to also contain cholecystokinin immunoreactivity. Neurons single-labeled for either peptide were also present, with those immunoreactive for cholecystokinin alone far outnumbering those containing only neurotensin. By combining the double-labeling colocalization technique with fluorescence retrograde tracing, some of the forebrain projections of these neurons were determined. Ventral mesencephalic neurons containing both neurotensin and cholecystokinin were found to project to the nucleus accumbens, prefrontal cortex, or amygdala. The present results, combined with those of previous studies, suggest that there are complex heterogeneous subpopulations of presumed dopaminergic ventral mesencephalic neurons which give rise to the ascending mesotelencephalic systems and which may contain both neurotensin and cholecystokinin, either peptide alone, or neither of these two peptides.
通过使用双标记间接免疫荧光法在同一组织切片中同时检测两种抗原，在大鼠腹侧中脑神经元中证实了神经降压素样免疫反应性和胆囊收缩素样免疫反应性的共定位。发现在整个腹侧中脑（主要位于腹侧被盖区、内侧黑质、以及头端和尾端中缝核）分布的超过90%的神经降压素阳性核周体也含有胆囊收缩素免疫反应性。也存在仅对一种肽呈单标记的神经元，其中仅对胆囊收缩素呈免疫反应性的神经元数量远多于仅含神经降压素的神经元。通过将双标记共定位技术与荧光逆行追踪相结合，确定了这些神经元的一些前脑投射。发现同时含有神经降压素和胆囊收缩素的腹侧中脑神经元投射到伏隔核、前额叶皮质或杏仁核。目前的结果与先前研究的结果相结合，表明假定的多巴胺能腹侧中脑神经元存在复杂的异质亚群，它们形成了中脑-间脑上行系统，并且可能同时含有神经降压素和胆囊收缩素、仅含其中一种肽或这两种肽都不含。
