Kalivas P W, Miller J S
Brain Res. 1984 May 21;300(1):157-60. doi: 10.1016/0006-8993(84)91351-9.
A high density of neurotensin-containing neuronal perikarya are found in the ventral tegmental area, and some of these neurons also contain dopamine. In this study, we used retrograde transport of horseradish peroxidase (HRP) coupled with immunohistochemical staining for neurotensin to determine whether ventral tegmental neurotensin neurons project to known dopamine terminal areas of the limbic forebrain. It was found that HRP injection into the medial nucleus accumbens and diagonal band of Broca resulted in neurons in the ventral and medial ventral tegmental area containing both HRP granules and neurotensin immunoreactivity.
在腹侧被盖区发现了高密度的含神经降压素的神经元胞体，其中一些神经元也含有多巴胺。在本研究中，我们使用辣根过氧化物酶（HRP）的逆行运输结合神经降压素的免疫组织化学染色，以确定腹侧被盖区的神经降压素神经元是否投射到边缘前脑已知的多巴胺终末区域。结果发现，将HRP注射到伏隔核内侧和布罗卡斜带后，腹侧和内侧腹侧被盖区的神经元同时含有HRP颗粒和神经降压素免疫反应性。
