Departamento de Zootecnia, Laboratório de Aquacultura, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 30161-970, Brazil.
Departamento de Fisiologia e Farmacologia, Laboratório de Fisiologia de Peixes, Universidade Federal de Santa Maria, Avenida Roraima, 1000, Santa Maria, RS, CEP 97105-900, Brazil.
Fish Physiol Biochem. 2021 Feb;47(1):135-152. doi: 10.1007/s10695-020-00900-x. Epub 2020 Nov 16.
This study aimed to evaluate the essential oil of Ocimum gratissimum L. (EOOG) for anesthesia and in the transport of Oreochromis niloticus. Experiment I determined the time of anesthesia induction and recovery during anesthesia of O. niloticus exposed to different concentrations of EOOG (0, 30, 90, 150, and 300 mg L). Based on data from Experiment I, Experiment II evaluated the effect of 0, 30, and 90 mg L EOOG on blood parameters and oxidative stress immediately after anesthesia induction and 1 h after recovery. Experiment III evaluated the effect of 0, 5, and 10 mg L EOOG on blood variables immediately after 4.5 h of transport of juveniles. Concentrations between 90 and 150 mg L EOOG were efficient for anesthesia and recovery. The use of 90 mg L of EOOG prevented an increase in plasma glucose. Other changes in blood parameters and oxidative stress are discussed. The use of 10 mg L EOOG in transport increased plasma glucose and decreased hematocrit values immediately after transport. It is concluded that the use of 90 and 150 mg L EOOG causes anesthesia and recovery in O. niloticus within the time intervals considered ideal. The use of 90 mg L EOOG favored stable plasma glucose soon after anesthesia induction and 1 h after recovery, but caused changes in the antioxidant defense system by increasing hepatic and kidney ROS. The transport of 12 g O. niloticus for 4.5 h can be performed with concentration of 5 mg L of EOOG.
本研究旨在评估奥图姆 gratissimum L. (EOOG) 精油在麻醉和奥尼罗非鱼运输中的作用。实验 I 确定了暴露于不同浓度 EOOG(0、30、90、150 和 300 mg L)的奥尼罗非鱼麻醉诱导和恢复时间。基于实验 I 的数据,实验 II 评估了 0、30 和 90 mg L EOOG 对麻醉诱导后即刻和恢复后 1 小时血液参数和氧化应激的影响。实验 III 评估了 0、5 和 10 mg L EOOG 对幼鱼运输 4.5 小时后血液变量的影响。90 至 150 mg L EOOG 浓度可有效麻醉和恢复。使用 90 mg L EOOG 可防止血浆葡萄糖升高。其他血液参数和氧化应激的变化将在讨论中进行讨论。在运输中使用 10 mg L EOOG 会立即增加血浆葡萄糖并降低红细胞压积值。结论是,在考虑的理想时间间隔内,使用 90 和 150 mg L EOOG 可使奥尼罗非鱼麻醉和恢复。使用 90 mg L EOOG 可在麻醉诱导后即刻和恢复后 1 小时内使血浆葡萄糖稳定,但通过增加肝和肾 ROS 导致抗氧化防御系统发生变化。可使用 5 mg L 的 EOOG 运输 12 克奥尼罗非鱼 4.5 小时。