Salbego J, Toni C, Becker A G, Zeppenfeld C C, Menezes C C, Loro V L, Heinzmann B M, Baldisserotto B
Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria - UFSM, Campus Camobi, Avenida Roraima, 1000, CEP 97105-900, Santa Maria, RS, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia Farroupilha, Campus Frederico Westphalen, Linha 7 de Setembro, s/n, CEP 98400-000, Frederico Westphalen, RS, Brazil.
Braz J Biol. 2017 Nov;77(4):696-702. doi: 10.1590/1519-6984.16515. Epub 2017 May 4.
The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 µL L-1) and essential oil of Lippia alba (EOL) (10 or 20 µL L-1) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L-1). The addition of various concentrations of EUG (1.5 or 3.0 µL L-1) and EOL (10 or 20 µL L-1) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 µL L-1) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.
活鱼运输是水产养殖中的常规操作，对动物而言是相当大的应激源。在用于鱼类运输的水中添加麻醉剂可预防或减轻运输应激的有害影响。本研究调查了添加丁香酚（EUG）（1.5或3.0 μL L-1）和白香蜂草精油（EOL）（10或20 μL L-1）对银鲶（Rhamdia quelen；Quoy和Gaimard，1824）肝脏和肌肉中的代谢参数（糖原、乳酸和总蛋白水平）、肌肉和大脑中的乙酰胆碱酯酶活性（AChE）以及肝脏中蛋白质羰基（PC）、硫代巴比妥酸反应性物质（TBARS）和非蛋白硫醇基团（NPSH）水平及谷胱甘肽-S-转移酶活性的影响。银鲶装在塑料袋中运输4小时（装载密度为169.2 g L-1）。向运输水中添加不同浓度的EUG（1.5或3.0 μL L-1）和EOL（10或20 μL L-1）对银鲶运输是可取的，因为这些物质的两种浓度均提高了肝脏中NPSH抗氧化剂水平并降低了TBARS水平。此外，与对照组相比，这些组肝脏中的糖原和乳酸水平较低，大脑中的AChE活性较低（EOL 10或20 μL L-1），这表明麻醉剂给药后能量代谢和神经传递较低，有助于维持体内稳态和镇静状态。
