处理和切片未脱钙的鼠骨标本。
Processing and Sectioning Undecalcified Murine Bone Specimens.
机构信息
Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
出版信息
Methods Mol Biol. 2021;2230:231-257. doi: 10.1007/978-1-0716-1028-2_14.
Preparation of mineralized tissue specimens for bone-specific staining encompasses a critical sequence of histological techniques that provides visualization of tissue and cellular morphology. Bone specimens are fixed in 10% neutral buffered formalin (NBF), dehydrated in graded ethanol (EtOH) solutions (and optionally cleared in xylene), infiltrated and embedded in polymethyl methacrylate (methyl methacrylate or MMA), classically sliced into 4-10 micrometer (μm) sections, and stained with bone-specific histological stains such as von Kossa (with either nuclear fast red solution counterstain or MacNeal's tetrachrome counterstain), modified Goldner's trichrome, Alizarin Red S, Safranin O, and tartrate-resistant acid phosphatase (TRAP) stain. Here, we describe the tissue processing of mineralized mouse bones from dissection to staining for histological analysis by light microscopy.
矿化组织标本的制备包括一系列关键的组织学技术，这些技术可以提供组织和细胞形态的可视化。骨标本用 10%中性缓冲福尔马林（NBF）固定，用梯度乙醇（EtOH）溶液（可选地用二甲苯透明）脱水，浸透并嵌入聚甲基丙烯酸甲酯（甲基丙烯酸甲酯或 MMA）中，经典地切成 4-10 微米（μm）的切片，并用骨特异性组织学染色剂染色，如 von Kossa（用核快速红溶液或 MacNeal 的四色对照染色）、改良 Goldner 的三色、茜素红 S、番红 O 和抗酒石酸酸性磷酸酶（TRAP）染色。在这里，我们描述了从小鼠骨骼解剖到通过光学显微镜进行组织学分析的矿化组织标本的处理过程。
Zotero 插件