Department of Biology, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Department of Biology, Brandon University, Brandon, MB R7A 6A9, Canada.
Plant Dis. 2021 Jan;105(1):127-133. doi: 10.1094/PDIS-06-20-1165-RE. Epub 2020 Nov 16.
Soybean () is one of the most economically important crops grown in North America and in other regions worldwide. However, the plant is susceptible to a variety of foliar pathogenic microorganisms, some of which are a significant threat to production. Several molecular and serological approaches are currently available to diagnose plant pathogens, but all have limitations including their capability to accurately detect coinfections of individual plants. We therefore used 16S and internal transcribed spacer amplicon sequencing to identify the suite of bacterial and fungal organisms infecting 96 soybean leaf samples collected throughout southern Manitoba, Canada, at two growth stages (V2/3 and R6). We were able to confirm the presence of pathogens previously known to our sampling regions, such as , , and spp. Importantly, we found that most of plants were infected by more than one putative pathogen, with 64% of V2/3 and 26% of R6 plants infected by three or more pathogens. Amplicon sequencing also indicated the presence of residual pathogens that infect crops other than soybean, as well as nonfoliar pathogens and nonpathogenic microorganisms. We discuss some of the benefits and drawbacks of using amplicon sequencing to detect foliar pathogens of soybean.
大豆是北美和世界其他地区种植的最重要的经济作物之一。然而，该植物易受各种叶部病原微生物的影响，其中一些对生产构成重大威胁。目前有几种分子和血清学方法可用于诊断植物病原体，但都有其局限性，包括准确检测单个植物的合并感染的能力。因此，我们使用 16S 和内部转录间隔区扩增子测序来鉴定在加拿大马尼托巴省南部两个生长阶段（V2/3 和 R6）采集的 96 个大豆叶片样本中感染的细菌和真菌生物。我们能够确认在我们的采样区域已知的病原体的存在，例如 、 和 spp. 重要的是，我们发现大多数植物受到一种以上假定病原体的感染，64%的 V2/3 植物和 26%的 R6 植物受到三种或更多病原体的感染。扩增子测序还表明存在感染除大豆以外的其他作物的残余病原体以及非叶部病原体和非致病性微生物。我们讨论了使用扩增子测序来检测大豆叶部病原体的一些优点和缺点。
