He Yunqiang, Li Yan, Song Yulin, Hu Xingming, Liang Jinbo, Shafik Karim, Ni Dejiang, Xu Wenxing
Hubei Hongshan Laboratory, Wuhan 430070, China.
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Wuhan 430070, China.
J Fungi (Basel). 2022 Jul 26;8(8):782. doi: 10.3390/jof8080782.
Amplicon sequencing is a powerful tool for analyzing the fungal composition inside plants, whereas its application for the identification of etiology for plant diseases remains undetermined. Here, we utilize this strategy to clarify the etiology responsible for tea leaf brown-black spot disease (LBSD), a noticeable disease infecting tea plants etiology that remains controversial. Based on the ITS-based amplicon sequencing analysis, species were identified as separate from spp. and sp., which are concluded as the etiological agents. This was further confirmed by the fungal isolation and their specific pathogenicity on diverse tea varieties. Based on the morphologies and phylogenetic analysis constructed with multi-loci (ITS, LSU, and ), two novel species-tentatively named and as reference to their host plants-were proposed and characterized. Here, we present an integrated approach of ITS-based amplicon sequencing in combination with fungal isolation and fulfillment of Koch's postulates for etiological identification of tea plant disease, revealing new etiology for LBSD. This contributes useful information for further etiological identification of plant disease based on amplicon sequencing, as well as understanding, prevention, and management of this economically important disease.
扩增子测序是分析植物体内真菌组成的有力工具，但其在植物病害病因鉴定中的应用仍未确定。在此，我们利用这一策略来阐明茶树叶片褐黑色斑病（LBSD）的病因，这是一种感染茶树的明显病害，其病因仍存在争议。基于基于ITS的扩增子测序分析，鉴定出了与 spp. 和 sp. 不同的物种，这些物种被确定为病原体。通过真菌分离及其对不同茶树品种的特定致病性进一步证实了这一点。基于多基因座（ITS、LSU、 和 ）构建的形态学和系统发育分析，提出并鉴定了两个新的 物种，根据其寄主植物暂命名为 和 。在此，我们提出了一种基于ITS的扩增子测序与真菌分离以及科赫法则验证相结合的综合方法，用于茶树病害的病因鉴定，揭示了LBSD的新病因。这为基于扩增子测序的植物病害进一步病因鉴定以及对这种经济上重要病害的理解、预防和管理提供了有用信息。
