Department of Biological Sciences, University of Toledo, 2801 West Bancroft Street, Mail Stop 601, Toledo, OH, 43606, USA.
USDA-Agricultural Research Service, Application Technology Research Unit, 2801 West Bancroft Street, Mail Stop 604, Toledo, OH, 43606, USA.
Virology. 2021 Jan 15;553:9-22. doi: 10.1016/j.virol.2020.10.003. Epub 2020 Oct 30.
During an infection, Cauliflower mosaic virus (CaMV) forms inclusion bodies (IBs) mainly composed of viral protein P6, where viral activities occur. Because viral processes occur in IBs, understanding the mechanisms by which they are formed is crucial. FL-P6 expressed in N. benthamiana leaves formed IBs of a variety of shapes and sizes. Small IBs were dynamic, undergoing fusion/dissociation events. Co-expression of actin-binding polypeptides with FL-P6 altered IB size distribution and inhibited movement. This suggests that IB movement is required for fusion and growth. A P6 deletion mutant was discovered that formed a single large IB per cell, which suggests it exhibited altered fusion/dissociation dynamics. Myosin-inhibiting drugs did not affect small IB movement, while those inhibiting actin polymerization did. Large IBs colocalized with components of the aggresome pathway, while small ones generally did not. This suggests a possible involvement of the aggresome pathway in large IB formation.
在感染过程中，芜菁花叶病毒（CaMV）形成包含体（IBs），主要由病毒蛋白 P6 组成，病毒活动发生在其中。由于病毒过程发生在 IBs 中，因此理解它们形成的机制至关重要。在 N. benthamiana 叶片中表达的 FL-P6 形成了各种形状和大小的 IBs。小 IBs 是动态的，经历融合/解离事件。与 FL-P6 共表达肌动蛋白结合多肽会改变 IB 的大小分布并抑制运动。这表明 IB 的运动对于融合和生长是必需的。发现一种 P6 缺失突变体，它在每个细胞中形成一个大的 IB，这表明它表现出改变的融合/解离动力学。肌球蛋白抑制剂药物不影响小 IB 的运动，而抑制肌动蛋白聚合的药物则会影响。大 IBs 与聚集体途径的成分共定位，而小 IBs 则通常不共定位。这表明聚集体途径可能参与大 IB 的形成。
