Kaneko Y, Hayashi N, Toh-e A, Banno I, Oshima Y
Institute for Fermentation, Osaka, Japan.
Gene. 1987;58(1):137-48. doi: 10.1016/0378-1119(87)90036-9.
The nucleotide sequence of a 3694-bp DNA fragment bearing the PHO8 gene encoding nonspecific repressible alkaline phosphatase (rALPase; EC 3.1.3.1) of Saccharomyces cerevisiae was determined. The sequence contains a 1698 bp open reading frame (ORF), and the major PHO8 transcription start point at 32 bp upstream from the ATG codon; several minor transcription start points are located between the major start point and ATG. The major start point is most responsive to the phosphate signals. The amino acid (aa) sequence deduced from the ORF contains several homologous regions in common with alkaline phosphatases of Escherichia coli and human placenta. A PHO8 DNA fragment previously isolated [Kaneko et al., Mol. Cell. Biol. 5 (1985) 248-252] was found to be truncated for the region encoding the 22 aa residues at the C terminus of the enzyme, which were replaced with 17 aa encoded by a pBR322 DNA. The modified gene could produce significant rALPase activity without the function of proteinase A which is required for the maturation of rALPase from its precursor.
测定了酿酒酵母中携带编码非特异性可阻遏碱性磷酸酶（rALPase；EC 3.1.3.1）的PHO8基因的3694bp DNA片段的核苷酸序列。该序列包含一个1698bp的开放阅读框（ORF），主要的PHO8转录起始点位于ATG密码子上游32bp处；几个次要转录起始点位于主要起始点和ATG之间。主要起始点对磷酸盐信号最敏感。从ORF推导的氨基酸（aa）序列包含与大肠杆菌和人胎盘碱性磷酸酶共有的几个同源区域。发现先前分离的PHO8 DNA片段[Kaneko等人，《分子细胞生物学》5（1985）248 - 252]在编码该酶C末端22个aa残基的区域被截短，被pBR322 DNA编码的17个aa取代。修饰后的基因在没有蛋白酶A功能的情况下也能产生显著的rALPase活性，而蛋白酶A是rALPase从前体成熟所必需的。
