Curso de Odontologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil.
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N, Av. W5, Campus II, Modulo C, room C-221, Brasília, Distrito Federal, 70790-160, Brazil.
Clin Oral Investig. 2021 Jun;25(6):3623-3632. doi: 10.1007/s00784-020-03684-9. Epub 2020 Nov 16.
In order to evaluate host defense peptides (HDPs) HHC-10 and synoeca-MP activity in in vitro osteoclastogenesis process and in vivo induced apical periodontitis, testing the effect of molecules in the inflammatory response and in apical periodontitis size/volume after root canal treatment.
In vitro osteoclastogenesis was assessed on bone marrow cell cultures extracted from mice, while in vivo endodontic treatment involved rats treated with Ca(OH) or HDPs. In vitro osteoclasts were subjected to TRAP staining, and in vivo samples were evaluated by radiographic and tomographic exams, as well as histologic analysis.
None of the substances downregulated the in vitro osteoclastogenesis. Nevertheless, all treatments affected the average of apical periodontitis size in rats, although only teeth treated with HDPs demonstrated lower levels of the inflammatory process. These results demonstrated the in vivo potential of HDPs. Radiographic analysis suggested that HHC-10 and synoeca-MP-treated animals presented a similar lesion size than Ca(OH)-treated animals after 7-day of endodontic treatment. However, tomography analysis demonstrated smaller lesion volume in synoeca-MP-treated animals than HHC-10 and Ca(OH)-treated animals, after 7 days.
These molecules demonstrated an auxiliary effect in endodontic treatment that might be related to its immunomodulatory ability, broad-spectrum antimicrobial activity, and possible induction of tissue repair at low concentrations. These results can encourage further investigations on the specific mechanisms of action in animal models to clarify the commercial applicability of these biomolecules for endodontic treatment.
HDPs have the potential to be adjuvant substances in endodontic therapy due to its potential to reduce inflammation in apical periodontitis.
为了评估宿主防御肽（HDPs）HHC-10 和 synoeca-MP 在体外破骨细胞生成过程中和在体内诱导根尖周炎中的活性，测试分子在炎症反应和根管治疗后根尖周炎大小/体积中的作用。
体外破骨细胞生成在从小鼠提取的骨髓细胞培养物上进行评估，而体内根管治疗涉及用 Ca(OH)或 HDPs 治疗的大鼠。体外破骨细胞进行 TRAP 染色，体内样本通过放射照相和断层扫描检查以及组织学分析进行评估。
没有一种物质能下调体外破骨细胞生成。然而，所有治疗均影响大鼠根尖周炎大小的平均值，尽管仅用 HDPs 治疗的牙齿显示出较低水平的炎症过程。这些结果表明 HDPs 的体内潜力。放射照相分析表明，在根管治疗后 7 天，用 HHC-10 和 synoeca-MP 治疗的动物的病变大小与用 Ca(OH)治疗的动物相似。然而，在 7 天后，断层扫描分析表明，在用 synoeca-MP 治疗的动物中，病变体积小于用 HHC-10 和 Ca(OH)治疗的动物。
这些分子在根管治疗中表现出辅助作用，这可能与其免疫调节能力、广谱抗菌活性和在低浓度下可能诱导组织修复有关。这些结果可以鼓励在动物模型中进一步研究其特定作用机制，以阐明这些生物分子在根管治疗中的商业应用潜力。
由于 HDPs 具有减轻根尖周炎炎症的潜力，因此有可能成为根管治疗的辅助物质。
