Department of Transfusion Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing, China.
NMPA Key Laboratory for Quality Control of Blood Products, Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, Beijing, China.
J Med Virol. 2021 Aug;93(8):4780-4785. doi: 10.1002/jmv.26666. Epub 2021 Mar 14.
Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) are known to infect humans and transmit through contaminated blood and blood products. Globally, three genotypes of B19V, as well as PARV4, have been identified, respectively. The existence of different B19V genotypes in Chinese plasma donors has been investigated, however, the data regarding PARV4 were not available. The main objective of this study is to identify the genotypes of PARV4 circulating in Chinese plasma donors. By using a duplex quantitative polymerase chain reaction assay adapted for all genotypes of B19V and PARV4, 78 source plasma pools for fractionation were screened and quantified. Results showed that positive rates of B19V and PARV4 DNA in plasma pool samples were 25.64% and 14.10%, respectively. PARV4 sequences in two positive samples were next genotyped, and these two sequences belonged to PARV4 genotypes 1 and 2, respectively. In conclusion, the data present demonstrate the existence of PARV4 genotypes 1 and 2 in Chinese plasma donors for the first time and also show the relatively lower prevalence and level of PARV4 DNA in Chinese plasma donors in comparison with that of B19V DNA.
人细小病毒 B19（B19V）和人细小病毒 4（PARV4）已知可感染人类，并通过受污染的血液和血液制品传播。在全球范围内，已分别鉴定出三种 B19V 基因型以及 PARV4。已经研究了中国血浆供体中不同 B19V 基因型的存在情况，但关于 PARV4 的数据不可用。本研究的主要目的是鉴定中国血浆供体中循环的 PARV4 基因型。通过使用适应所有 B19V 和 PARV4 基因型的双重定量聚合酶链反应检测法，对用于分离的 78 个源血浆池进行了筛选和定量。结果显示，血浆池样本中 B19V 和 PARV4 DNA 的阳性率分别为 25.64%和 14.10%。对两个阳性样本中的 PARV4 序列进行了下一代基因分型，这两个序列分别属于 PARV4 基因型 1 和 2。总之，这些数据首次证明了中国血浆供体中存在 PARV4 基因型 1 和 2，并且与 B19V DNA 相比，中国血浆供体中 PARV4 DNA 的流行率和水平相对较低。
