Department of Biochemistry, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Prof Egas Moniz, 1649-028, Lisboa, Portugal.
Proteomics and Protein Chemistry Unit, Department of Experimental and Health Sciences, Pompeu Fabra University, Dr Aiguader 88, Barcelona Biomedical Research Park, 08003, Barcelona, Spain.
Angew Chem Int Ed Engl. 2021 Jan 25;60(4):1686-1688. doi: 10.1002/anie.202006372. Epub 2020 Nov 17.
Proteolytic stability assessment is increasingly viewed as a fundamental component of peptide characterization, arguably of comparable importance as efficacy and toxicity data. A literature survey over the last decade reveals steady growth in the stability information available. However, it also uncovers two significant problems that hinder proper data comparison: 1) the use of different stability assays, and 2) the differences in how stability information is reported. In this Viewpoint, we present results from a database meta-analysis as well as concerns about the stability assessments published so far. We also suggest guidelines for a proper discussion between experts in the field on how to improve data readability so that peptide stability, an often-missing parameter in older literature, is adequately reported to take maximum advantage of it.
蛋白水解稳定性评估越来越被视为肽特性分析的基本组成部分，可以说与功效和毒性数据具有同等重要性。过去十年的文献调查显示，稳定性信息的可用数量稳步增长。然而，它也揭示了两个阻碍正确数据比较的重大问题：1）使用不同的稳定性测定法，以及 2）报告稳定性信息的方式不同。在本观点中，我们展示了数据库荟萃分析的结果以及对迄今为止发表的稳定性评估的关注。我们还建议了专家之间进行适当讨论的指南，以提高数据可读性，从而充分报告肽稳定性，这是旧文献中经常缺失的参数，以充分利用它。
