Mair Florian, Liechti Thomas
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, Washington, USA.
ImmunoTechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland, USA.
Cytometry A. 2021 Mar;99(3):231-242. doi: 10.1002/cyto.a.24269. Epub 2020 Dec 22.
Professional antigen-presenting cells (APCs), which include dendritic cells (DCs) and monocytes are essential for inducing and steering adaptive T-cell responses. Recent technological developments in single-cell analysis have significantly advanced our understanding of APC subset heterogeneity. To accurately resolve this functional diversity and to account for tissue-specific adaptation, novel phenotyping markers have been described more recently. While some of these largely overlap with traditionally used markers, more fine-grained phenotyping might be essential during inflammatory settings, where the traditional distinction between monocytes and dendritic cells has become blurred. Within this phenotype report, we provide a concise overview of traditional and recently described markers for the phenotyping of DCs and monocytes in the human system.
专业抗原呈递细胞（APC），包括树突状细胞（DC）和单核细胞，对于诱导和引导适应性T细胞反应至关重要。单细胞分析的最新技术发展显著推进了我们对APC亚群异质性的理解。为了准确解析这种功能多样性并考虑组织特异性适应性，最近描述了新的表型标记。虽然其中一些与传统使用的标记有很大重叠，但在炎症环境中，更精细的表型分析可能至关重要，因为在这种情况下，单核细胞和树突状细胞之间的传统区分已经变得模糊。在本表型报告中，我们简要概述了人类系统中用于DC和单核细胞表型分析的传统和最近描述的标记。
