Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0028, South Africa.
China Eucalypt Research Centre, Chinese Academy of Forestry, ZhanJiang 524022, GuangDong Province, China.
Plant Dis. 2021 Jun;105(6):1648-1656. doi: 10.1094/PDIS-05-20-1050-RE. Epub 2021 May 5.
The fungal pathogen, , has caused serious diseases of many important plants worldwide. Understanding the genetic diversity and mating type distribution of this pathogen provides an essential step toward the development of disease control measures. In this study, we designed 15 polymorphic microsatellite markers by using genome sequences of two isolates having opposite mating type and from different countries. These markers were used to determine the genetic diversity of 145 isolates representing 13 different hosts (12 plant hosts residing in 12 genera, and soil) from 10 countries. In addition, mating type genes were amplified to investigate the reproduction mode of the pathogens in these populations by using mating type primers designed for spp. Results revealed that a single dominant genotype, isolated from 11 plant genera residing in eight families, was present in seven countries across five continents. Only mating type MAT1-1 or MAT1-2 was amplified in each of the isolates, confirming that is heterothallic. Both mating types were detected in isolates from in South Africa and Uruguay. The MAT1-2 phenotype was widely distributed in isolates from 12 different hosts (11 plant hosts and soil) collected in 10 countries. Overall, the results suggest that there has been substantial global movement of and that this has shaped its current population structure.
真菌病原体 ，已在全球范围内对许多重要植物造成严重疾病。了解该病原体的遗传多样性和交配型分布，是制定疾病防控措施的重要一步。本研究利用来自不同国家的具有相反交配型的两个 分离株的基因组序列，设计了 15 个多态性微卫星标记。这些标记用于确定代表来自 10 个国家的 13 个不同宿主（12 个植物宿主分别属于 12 个属，以及土壤）的 145 个分离株的遗传多样性。此外，还扩增了交配型基因，利用为 spp.设计的交配型引物，调查这些种群中病原体的繁殖模式。结果表明，在来自五个大陆七个国家的 11 个植物属中存在单一的优势基因型。每个分离株中仅扩增到 MAT1-1 或 MAT1-2 交配型，证实 是异宗配合的。在南非和乌拉圭的分离株中均检测到两种交配型。MAT1-2 表型广泛分布于来自 10 个国家的 12 个不同宿主（11 个植物宿主和土壤）的分离株中。总的来说，研究结果表明 已经在全球范围内大量传播，这塑造了其当前的种群结构。
