Almeida Matheus Rocha, Horta Jacques Gabriel Álvares, de Matos Natália Alves, de Souza Ana Beatriz Farias, Castro Thalles de Freitas, Cândido Leandro da Silva, Andrade Mônica Campos, Cangussú Sílvia Dantas, Costa Guilherme de Paula, Talvani André, Bezerra Frank Silva
Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences (ICEB), Federal University of Ouro Preto (UFOP), Brazil.
Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences (ICEB), Federal University of Ouro Preto (UFOP), Brazil; Department of Clinical Medicine/Pediatrics, School of Medicine, Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
Respir Physiol Neurobiol. 2021 Feb;284:103583. doi: 10.1016/j.resp.2020.103583. Epub 2020 Nov 14.
This study aimed to analyze the effects of volume-controlled ventilation (VCV) and pressure-controlled ventilation (PCV) modes in female Wistar rats. 18 Wistar female adult rats were divided into three groups: control (CG), pressure-controlled ventilation (PCVG), and volume-controlled ventilation (VCVG). PCVG and VCVG were submitted to MV for one hour with a tidal volume (TV) of 8 mL/Kg, respiratory rate of 80 breaths/min, and positive end-expiratory pressure of 0 cmHO. At the end of the experiment, all animals were euthanized. The neutrophils and lymphocytes influx to lung were higher in VCVG and PCVG compared to CG. The activities of superoxide dismutase, catalase and myeloperoxidase were higher in PCVG compared to CG. There was an increase in lipid peroxidation and protein oxidation in PCVG compared to CG. The levels of CCL3 and CCL5 were higher in PCVG compared to CG. In conclusions, the PCV mode promoted structural changes in the lung parenchyma, redox imbalance and inflammation in healthy adult female rats submitted to MV.
本研究旨在分析容量控制通气（VCV）和压力控制通气（PCV）模式对雌性Wistar大鼠的影响。18只成年雌性Wistar大鼠被分为三组：对照组（CG）、压力控制通气组（PCVG）和容量控制通气组（VCVG）。PCVG组和VCVG组接受机械通气1小时，潮气量（TV）为8 mL/kg，呼吸频率为80次/分钟，呼气末正压为0 cmH₂O。实验结束时，所有动物均被安乐死。与CG组相比，VCVG组和PCVG组肺内中性粒细胞和淋巴细胞浸润更高。与CG组相比，PCVG组超氧化物歧化酶、过氧化氢酶和髓过氧化物酶的活性更高。与CG组相比，PCVG组脂质过氧化和蛋白质氧化增加。与CG组相比，PCVG组CCL3和CCL5水平更高。总之，PCV模式在接受机械通气的健康成年雌性大鼠中促进了肺实质的结构变化、氧化还原失衡和炎症反应。
