Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences (ICEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Laboratory of Immunobiology of Inflammation (LABIIN), Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences (ICEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Exp Biol Med (Maywood). 2020 Sep;245(15):1404-1413. doi: 10.1177/1535370220940995. Epub 2020 Jul 8.
Mechanical ventilation (MV) is a tool used for the treatment of patients with acute or chronic respiratory failure. However, MV is a non-physiological resource, and it can cause metabolic disorders such as release of pro-inflammatory cytokines and production of reactive oxygen species. In clinical setting, maneuvers such as sigh, are used to protect the lungs. Thus, this study aimed to evaluate the effects of sigh on oxidative stress and lung inflammation in healthy adult Wistar rats submitted to MV. Male Wistar rats were divided into four groups: control (CG), mechanical ventilation (MVG), MV set at 20 sighs/h (MVG20), and MV set at 40 sighs/h (MVG40). The MVG, MVG20, and MVG40 were submitted to MV for 1 h. After the protocol, all animals were euthanized and the blood, bronchoalveolar lavage fluid, and lungs were collected for subsequent analysis. In the arterial blood, MVG40 presented higher partial pressure of oxygen and lower partial pressure of carbon dioxide compared to control. The levels of bicarbonate in MVG20 were lower compared to CG. The neutrophil influx in bronchoalveolar lavage fluid was higher in the MVG compared to CG and MVG40. In the lung parenchyma, the lipid peroxidation was higher in MVG compared to CG, MVG20, and MVG40. Superoxide dismutase and catalase activity were higher in MVG compared to CG, MVG20, and MVG40. The levels of IL-1, IL-6, and TNF in the lung homogenate were higher in MVG compared to CG, MVG20, and MVG40. The use of sigh plays a protective role as it reduced redox imbalance and pulmonary inflammation caused by MV.
机械通气（MV）是治疗急性或慢性呼吸衰竭患者的一种工具。然而，MV 是一种非生理资源，它会引起代谢紊乱，如促炎细胞因子的释放和活性氧的产生。在临床环境中，使用叹气等操作来保护肺部。因此，本研究旨在评估在健康成年 Wistar 大鼠中使用叹气对 MV 引起的氧化应激和肺部炎症的影响。雄性 Wistar 大鼠分为四组：对照组（CG）、机械通气组（MVG）、设置为 20 次/小时叹气的 MV 组（MVG20）和设置为 40 次/小时叹气的 MV 组（MVG40）。MVG、MVG20 和 MVG40 均接受 MV 治疗 1 小时。方案结束后，所有动物均被安乐死，采集血液、支气管肺泡灌洗液和肺组织进行后续分析。在动脉血中，MVG40 组的氧分压较高，二氧化碳分压较低。MVG20 组的碳酸氢盐水平较 CG 组低。支气管肺泡灌洗液中的中性粒细胞浸润在 MVG 组较 CG 组和 MVG40 组高。在肺实质中，MVG 组的脂质过氧化水平较 CG 组、MVG20 组和 MVG40 组高。超氧化物歧化酶和过氧化氢酶活性在 MVG 组较 CG 组、MVG20 组和 MVG40 组高。肺组织匀浆中的 IL-1、IL-6 和 TNF 水平在 MVG 组较 CG 组、MVG20 组和 MVG40 组高。叹气的使用起到了保护作用，因为它减轻了 MV 引起的氧化还原失衡和肺部炎症。
