Agricultural Bio-pharmaceutical Laboratory, Qingdao Agricultural University, Qingdao, 266109, China.
National Centre for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, Agricultural University, Beijing, 100089, China.
BMC Complement Med Ther. 2020 Nov 17;20(1):347. doi: 10.1186/s12906-020-03090-4.
The aerial parts of Salvia miltiorrhiza, which was considered to be the waste part and discarded during the root harvest, is rich in protocatechuic aldehyde (PAI). This study investigated the health-promoting effects of extracts and PAI from the aerial parts of Salvia miltiorrhiza, including its anti-inflammatory effects and the underlying mechanisms of action in vitro and in vivo.
Purification of the sample paste of Salvia miltiorrhiza was accomplished using HPLC analysis. TheMTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay was employed to determine the cell viability. The production of inflammatory factors was detected by ELISA assays. The histopathological analysis was used to analyse the lungs and livers of mice treated with PAI. Western blot was performed to reveal the mechanism of PAI in anti-inflammatory.
The extracts and PAI from the aerial parts of Salvia miltiorrhiza inhibited TNF-α, IL-6 production and promoted the production of IL-10 in vivo in mice and in vitro in the macrophage cell line RAW264.7. NF-κB and MAPKs kinase phosphorylation were also suppressed by PAI in vivo and in vitro, indicating that PAI exhibited an anti-inflammatory effect.
These findings suggest that the aerial parts of Salvia miltiorrhiza extract may serve as potential protective agents for inflammatory.
丹参的地上部分曾被视为根收获过程中的废弃物而丢弃，但富含原儿茶醛（PAI）。本研究旨在探讨丹参地上部分提取物及 PAI 的促健康作用，包括其在体外和体内的抗炎作用及其作用机制。
采用 HPLC 分析对丹参样品糊进行纯化。MTT（噻唑蓝）法测定细胞活力。ELISA 法检测炎性因子的产生。对给予 PAI 的小鼠的肺和肝进行组织病理学分析。Western blot 揭示 PAI 的抗炎作用机制。
丹参地上部分提取物及 PAI 抑制 TNF-α、IL-6 的产生，促进体内小鼠和体外巨噬细胞 RAW264.7 中 IL-10 的产生。PAI 还抑制体内和体外的 NF-κB 和 MAPKs 激酶磷酸化，表明 PAI 具有抗炎作用。
这些发现表明丹参地上部分提取物可能是炎症的潜在保护剂。
