State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
Carbohydr Polym. 2017 Aug 1;169:304-314. doi: 10.1016/j.carbpol.2017.04.034. Epub 2017 Apr 17.
We report here the discovery of a polysaccharide, namely EUP1, with anti-inflammatory activity from the herb of Eucommia ulmoides Oliv. We separated three polysaccharide fractions from this herb based on acidity and screened them for their activity in modulating the phenotype of murine macrophages. Among them, EUP1 was the only fraction to exert such a function - it stimulated Raw 264.7 cells to express CD206 and a key anti-inflammatory cytokine interleukin-10. Having fully characterised EUP1 with a series of chromatographic and spectroscopic analyses, we evaluated its anti-inflammatory effects in both in vitro and in vivo inflammatory models. In the murine model of sepsis induced by lipopolysaccharide, administration of EUP1 effectively suppressed the expression of major inflammatory cytokines, alleviated lung injury and increased animal survival rate. In summary, EUP1, with a clearly elucidated chemical structure and solid anti-inflammatory activity, may become a valuable candidate for further development into an anti-septic therapeutic agent.
我们在这里报告了从杜仲植物中发现的一种具有抗炎活性的多糖，即 EUP1。我们根据酸度从这种草药中分离出三种多糖级分，并筛选它们调节小鼠巨噬细胞表型的活性。其中，EUP1 是唯一具有这种功能的级分 - 它刺激 Raw 264.7 细胞表达 CD206 和关键的抗炎细胞因子白细胞介素-10。通过一系列色谱和光谱分析对 EUP1 进行了充分表征后，我们在体外和体内炎症模型中评估了其抗炎作用。在脂多糖诱导的脓毒症小鼠模型中，EUP1 的给药有效抑制了主要炎症细胞因子的表达，减轻了肺损伤并提高了动物的存活率。总之，EUP1 具有明确阐明的化学结构和可靠的抗炎活性，可能成为进一步开发成为抗脓毒症治疗剂的有价值的候选药物。
