Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
J Sci Food Agric. 2021 May;101(7):3013-3020. doi: 10.1002/jsfa.10935. Epub 2020 Nov 26.
Plant secondary metabolites, including tannins, saponins and phenolic acids, possess potential methane (CH ) inhibition bioactivity. Caffeic acid (CA), as one of the typical phenolic acids, serves as a promising rumen CH inhibitor, but the underlying mechanisms and investigations with typical formulated rations are still not well documented. Therefore, a batch culture study was conducted to investigate the effects of CA on methanogenesis, rumen fermentation and growth of ruminal microorganisms when high-forage or high-concentrate substrates are fermented.
After 48 h incubations, adding CA up to 40 g kg dry matter linearly reduced (P < 0.05) the disappearance of dry matter, neutral detergent fiber (NDFD), total gas, methanogenesis, total volatile fatty acid and 16S rDNA copy numbers of Ruminococcus albus and Butyrivibrio fibrisolvens, and increased 16S rDNA copy numbers of methanogens for the high-forage treatment. For the high-concentrate treatment, CA exerted opposite effects (P < 0.05) on the above variables, except that CA did not affect (P > 0.05)16S rDNA copy numbers of methanogens or R. albus.
Caffeic acid inhibited in vitro methanogenesis and rumen fermentation with high-forage substrate incubation. Contrarily, CA benefited in vitro fermentation and enhanced methanogenesis with high-concentrate substrate incubation. It suggests that CA modulates methanogenesis and rumen fermentation mainly by affecting the growth of cellulolytic bacteria in vitro. © 2020 Society of Chemical Industry.
植物次生代谢物，包括单宁、皂苷和酚酸，具有抑制甲烷（CH ）的生物活性。咖啡酸（CA）作为典型的酚酸之一，是一种很有前途的瘤胃 CH 抑制剂，但潜在的作用机制和典型配方日粮的研究仍不充分。因此，进行了分批培养研究，以研究 CA 对高粗料或高精料发酵时甲烷生成、瘤胃发酵和瘤胃微生物生长的影响。
在 48 h 孵育后，添加 CA 高达 40 g kg 干物质线性降低（P < 0.05）干物质、中性洗涤纤维（NDFD）、总气体、甲烷生成、总挥发性脂肪酸和 Ruminococcus albus 的 16S rDNA 拷贝数和 Butyrivibrio fibrisolvens，并增加了高粗料处理中甲烷菌的 16S rDNA 拷贝数。对于高浓缩物处理，CA 对上述变量产生相反的影响（P < 0.05），除了 CA 对甲烷菌或 R. albus 的 16S rDNA 拷贝数没有影响（P > 0.05）。
CA 抑制高粗料底物孵育时的体外甲烷生成和瘤胃发酵。相反，CA 有利于高浓缩物底物孵育时的体外发酵和甲烷生成。这表明 CA 通过影响体外纤维分解菌的生长来调节甲烷生成和瘤胃发酵。© 2020 化学工业协会。
