Department of Bioengineering, Izmir Institute of Technology, Urla, Izmir, Turkey.
Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, Turkey.
Biotechnol Bioeng. 2021 Mar;118(3):1127-1140. doi: 10.1002/bit.27631. Epub 2020 Nov 28.
Tissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold-based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold-free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label-free magnetic levitation technique was used to form three-dimensional (3D) scaffold-free adipocyte structures with various fabrication strategies in a microcapillary-based setup. Adipogenic-differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.
组织工程研究旨在修复受损组织的形态和/或功能。组织工程研究主要依赖于支架技术。然而，这些技术存在一定的挑战，如选择合适的支架材料，包括机械性能、灭菌和制造工艺。作为一种替代方法，我们提出了一种基于磁悬浮的新型无支架脂肪组织生物制造技术。在这项研究中，使用无标记的磁悬浮技术，在基于微毛细管的设置中使用各种制造策略形成三维（3D）无支架脂肪细胞结构。使用成脂分化的 7F2 细胞和生长 D1 ORL UVA 干细胞作为模型细胞。分析了单细胞和共培养细胞的 3D 结构的形态特性。所开发的程序导致在没有支架的情况下形成不同模式的单细胞和共培养脂肪细胞。我们的结果表明，在磁悬浮平台上进行 3D 培养时，脂肪细胞形成松散的结构，而生长细胞则紧密堆积。该系统有望用于药物测试和细胞治疗中软组织损伤的移植应用的体外脂肪组织建模。此外，还可以将该技术扩展到其他细胞和组织类型。
