Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea.
Plant Mol Biol. 2021 Mar;105(4-5):365-383. doi: 10.1007/s11103-020-01093-w. Epub 2020 Nov 18.
Enhanced glutathione content improves lateral root development by positively regulating the transcripts of root development genes responsive to glutathione treatment, thereby increasing the overall productivity of rice plants. Glutathione is primarily known as a cellular antioxidant molecule, but its role in lateral root development in rice plants has not been elucidated. Here, we have investigated its role in lateral root development of rice Oryza sativa L. Exogenous glutathione (GSH) promoted both the number and length of lateral roots in rice, and the GSH biosynthesis inhibitor buthionine sulfoximine (BSO) significantly reduced these parameters, compared to untreated plants. The inhibition by BSO was reversed with exogenous GSH. Transcript profiling by RNA-seq revealed that expression of the transcription factor genes DREB and ERF and the hormone-related genes AOS, LOX, JAZ, and SAUR were significantly downregulated in the BSO-treated plants and, in contrast, upregulated in plants treated with GSH and with GSH and BSO together. We generated OsGS-overexpressing transgenic plants in which the transgene is controlled by the abiotic-stress-inducible OsRab21 promoter to study the effect of endogenously increased GSH levels. In cold stress, transgenic rice plants enhanced stress tolerance and lateral root development by maintaining redox homeostasis and improving upregulating the expression of transcription factors and hormone-related genes involved in lateral root development. We observed improved root growth of OsGS-overexpressing plants in paddy fields compared to the wild-type controls. These traits may have alleviated transplanting stress during early growth in the field and accounted for the increased productivity. These results provide information and perspectives on the role of GSH in gene expression, lateral root development, and grain yield in rice.
增强的谷胱甘肽含量通过正向调节对谷胱甘肽处理有反应的根发育基因的转录本，从而增加水稻植株的整体生产力，来改善侧根发育。谷胱甘肽主要作为一种细胞抗氧化分子而为人所知，但它在水稻侧根发育中的作用尚未阐明。在这里，我们研究了它在水稻(Oryza sativa L.)侧根发育中的作用。外源谷胱甘肽(GSH)促进了水稻侧根的数量和长度的增加，而谷胱甘肽生物合成抑制剂丁硫氨酸亚砜(BSO)与未处理的植物相比，显著降低了这些参数。用外源 GSH 逆转了 BSO 的抑制作用。通过 RNA-seq 进行的转录组分析表明，转录因子基因 DREB 和 ERF 以及激素相关基因 AOS、LOX、JAZ 和 SAUR 的表达在 BSO 处理的植物中显著下调，而在 GSH 处理的植物和 GSH 与 BSO 一起处理的植物中上调。我们生成了过表达 OsGS 的转基因植物，其中转基因受非生物胁迫诱导的 OsRab21 启动子控制，以研究内源性增加 GSH 水平的影响。在冷胁迫下，转基因水稻通过维持氧化还原平衡和提高参与侧根发育的转录因子和激素相关基因的表达来增强对胁迫的耐受性和侧根发育。与野生型对照相比，我们观察到过表达 OsGS 的植物在稻田中的根系生长得到了改善。这些特性可能减轻了早期田间生长中的移栽胁迫，并提高了生产力。这些结果提供了关于 GSH 在基因表达、侧根发育和水稻产量中的作用的信息和观点。
