Klepeckienė Kamilė, Radzijevskaja Jana, Ražanskė Irma, Žukauskienė Judita, Paulauskas Algimantas
Vytautas Magnus University, Kaunas, Lithuania.
J Vector Ecol. 2020 Dec;45(2):211-219. doi: 10.1111/jvec.12392.
The objectives of this study were to investigate the prevalence and abundance of deer keds on various cervids in Lithuania, to molecularly characterize the deer ked species based on mitochondrial COI and 16S rRNA genes, and to compare them with Lipoptena species found in other countries. A total of 11,939 deer keds (Lipoptena cervi and Lipoptena fortisetosa) was collected from the fur of 30 cervids in Lithuania between 2015 and 2019. The values of infestation with deer keds differed among the species of the hosts. Moose and red deer were more frequently infested with L. cervi than with L. fortisetosa, while L. fortisetosa was found more often on roe deer. Phylogenetic analysis of the COI and 16S rRNA genes of five Lipoptena species revealed 110 and 55 variable nucleotide positions, respectively. Among Lithuanian samples, three COI haplotypes of L. cervi and three haplotypes of L. fortisetosa were detected, while there was no variation observed in the 16S rRNA sequences analyzed with one haplotype of L. cervi and one haplotype of L. fortisetosa. This is the first study on L. cervi and L. fortisetosa parasitizing cervids and the first molecular characterization of these deer ked species in Lithuania.
本研究的目的是调查立陶宛各种鹿科动物身上鹿虱蝇的流行情况和丰度，基于线粒体细胞色素氧化酶亚基I（COI）和16S核糖体RNA（rRNA）基因对鹿虱蝇物种进行分子特征分析，并将它们与在其他国家发现的驼鹿虱属物种进行比较。2015年至2019年期间，在立陶宛从30只鹿科动物的皮毛上总共收集了11939只鹿虱蝇（鹿虱蝇和强鬃鹿虱蝇）。鹿虱蝇的感染率在宿主物种之间存在差异。驼鹿和马鹿感染鹿虱蝇的频率高于强鬃鹿虱蝇，而强鬃鹿虱蝇在狍子身上更为常见。对五个驼鹿虱属物种的COI和16S rRNA基因进行系统发育分析，分别揭示了110个和55个可变核苷酸位点。在立陶宛的样本中，检测到鹿虱蝇的三种COI单倍型和强鬃鹿虱蝇的三种单倍型，而在用鹿虱蝇的一种单倍型和强鬃鹿虱蝇的一种单倍型分析的16S rRNA序列中未观察到变异。这是关于寄生于鹿科动物的鹿虱蝇和强鬃鹿虱蝇的首次研究，也是立陶宛这些鹿虱蝇物种的首次分子特征分析。
