Group of Translational Research in Respiratory Medicine, Hospital Universitari Arnau de Vilanova y Santa Maria, IRB, Lleida, Spain.
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, Spain.
Respiration. 2020;99(12):1122-1128. doi: 10.1159/000511093. Epub 2020 Nov 18.
Obstructive sleep apnea (OSA) is a common disease caused by repeated episodes of collapse of the upper airway during sleep and is associated with the development of cardiovascular disease (CVD). However, there is high heterogeneity in the impact of OSA on patients. Until now, the profile of OSA patients at risk of developing CVD has not been defined, including the measurable variables that could be used to predict the CVD risk of a patient with OSA.
The aim of this study was to identify the microRNA (mi-RNA) profile associated with CVD in patients with OSA.
This is an observational, cross-sectional study that included 132 male patients. Three groups were defined as OSA patients, OSA patients with hypertension, and OSA patients who developed a major cardiovascular event. Polysomnography and ambulatory blood pressure measurements were performed. The expression profiling of 188 miRNAs in plasma was performed in 21 subjects (matched by BMI and age) by the TaqMan low density array (TLDA). miRNAs differentially expressed in the different subgroups of patients and miRNAs that correlated with the cardiovascular risk SCORE were selected for validation by RT-qPCR in the 111 remaining patients.
From the TLDA analysis, 7 miRNAs were selected for validation. Differential expression was not confirmed in any of the miRNAs. miR-143 was associated with nocturnal systolic blood pressure. miR-107 correlated with 24-h blood pressure parameters and with nocturnal hypertension. miR-486 was associated with the cardiovascular risk SCORE.
The circulating profile of miRNAs does not seem to be different in any of the subgroups of patients with OSA and different cardiovascular risk factors. Nevertheless, miR-107 and miR-143 are associated with specific blood pressure parameters in patients with OSA and miR-486 is associated with the cardiovascular risk SCORE.
阻塞性睡眠呼吸暂停（OSA）是一种常见疾病，由睡眠期间上呼吸道反复塌陷引起，并与心血管疾病（CVD）的发展有关。然而，OSA 对患者的影响存在很大的异质性。到目前为止，尚未确定发生 CVD 风险的 OSA 患者的特征，包括可用于预测 OSA 患者 CVD 风险的可测量变量。
本研究旨在确定与 OSA 患者 CVD 相关的 microRNA（miRNA）谱。
这是一项观察性、横断面研究，纳入了 132 名男性患者。将三组定义为 OSA 患者、伴有高血压的 OSA 患者和发生重大心血管事件的 OSA 患者。进行多导睡眠图和动态血压测量。通过 TaqMan 低密度阵列（TLDA）在 21 名（按 BMI 和年龄匹配）患者中进行血浆中 188 种 miRNA 的表达谱分析。选择不同亚组患者中差异表达的 miRNA 和与心血管风险 SCORE 相关的 miRNA，通过 RT-qPCR 在其余 111 名患者中进行验证。
从 TLDA 分析中选择了 7 种 miRNA 进行验证。在任何 miRNA 中都没有确认差异表达。miR-143 与夜间收缩压相关。miR-107 与 24 小时血压参数和夜间高血压相关。miR-486 与心血管风险 SCORE 相关。
OSA 患者和不同心血管危险因素的任何亚组中，循环 miRNA 谱似乎没有差异。然而，miR-107 和 miR-143 与 OSA 患者特定的血压参数相关，miR-486 与心血管风险 SCORE 相关。
